Osteoinductive silk-silica composite biomaterials for bone regeneration

被引：116

作者：

Mieszawska, Aneta J. ^{[1
]}

Fourligas, Nikolaos ^{[1
]}

Georgakoudi, Irene ^{[1
]}

Ouhib, Nadia M. ^{[2
]}

Belton, David J. ^{[3
]}

Perry, Carole C. ^{[3
]}

Kaplan, David L. ^{[1
]}

机构：

[1] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA

[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[3] Nottingham Trent Univ, Interdisciplinary Biomed Res Ctr, Sch Sci & Technol, Nottingham NG11 8NS, England

来源：

BIOMATERIALS | 2010年 / 31卷 / 34期

基金：

美国国家科学基金会;

关键词：

Silk; Silica; Osteogenesis; Nanoparticles; Composite; Stem cells; BOMBYX-MORI SILK; IN-VITRO; BIOACTIVE GLASSES; OSTEOGENIC DIFFERENTIATION; GENE-EXPRESSION; FIBROIN; COLLAGEN; DEGRADATION; FILMS; NANOPARTICLES;

D O I：

10.1016/j.biomaterials.2010.07.109

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Osteoinductive and biodegradable composite biomaterials for bone regeneration were prepared by combining silk fibroin with silica particles. The influence of these composite systems on osteogenesis was evaluated with human mesenchymal stem cells (hMSCs) subjected to osteogenic differentiation. hMSCs adhered, proliferated, and differentiated towards osteogenic lineages on silk/silica films. The addition of the silica to the silk films influenced gene expression leading to upregulation of bone sialoprotein (BSP) and collagen type 1 (Coil) osteogenic markers. Evidence for early bone formation in the form of collagen fibers and apatite nodules was obtained on the silk/silica films. Collagen fibers were closely associated with apatite deposits and overall collagen content was higher for the silica containing samples. Also, smaller sized silica particles (24 nm-2 mu m) with large surface area facilitated silica biodegradation in vitro through particle dissolution, leading to similar to 5-fold decrease in silica content over 10 weeks. These results indicate the suitability of silk/silica composite system towards bone regeneration, where degradation/remodeling rates of the organic and inorganic components can be controlled. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：8902 / 8910

页数：9

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