Environmental DNA facilitates accurate, inexpensive, and multiyear population estimates of millions of anadromous fish

被引:31
作者
Pochardt, Meredith [1 ,2 ,3 ]
Allen, Jennifer M. [1 ]
Hart, Ted [2 ]
Miller, Sophie D. L. [4 ]
Yu, Douglas W. [4 ,5 ,6 ]
Levi, Taal [1 ]
机构
[1] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA
[2] Chilkoot Indian Assoc, Haines, AK USA
[3] Takshanuk Watershed Council, Haines, AK USA
[4] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming, Yunnan, Peoples R China
[5] Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China
[6] Univ East Anglia, Sch Biol Sci, Norwich, Norfolk, England
基金
中国国家自然科学基金;
关键词
Alaska; anadromous; eDNA; eulachon; fisheries; Thaleichthys pacificus; Tlingit; EULACHON; EDNA; RARE;
D O I
10.1111/1755-0998.13123
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although environmental DNA shed from an organism is now widely used for species detection in a wide variety of contexts, mobilizing environmental DNA for management requires estimation of population size and trends in addition to assessing presence or absence. However, the efficacy of environmental-DNA-based indices of abundance for long-term population monitoring have not yet been assessed. Here we report on the relationship between six years of mark-recapture population estimates for eulachon (Thaleichthys pacificus) and "eDNA rates" which are calculated from the product of stream flow and DNA concentration. Eulachon are a culturally and biologically important anadromous fish that have significantly declined in the southern part of their range but were historically rendered into oil and traded. Both the peak eDNA rate and the area under the curve of the daily eDNA rate were highly predictive of the mark-recapture population estimate, explaining 84.96% and 92.53% of the deviance, respectively. Even in the absence of flow correction, the peak of the daily eDNA concentration explained an astonishing 89.53% while the area under the curve explained 90.74% of the deviance. These results support the use of eDNA to monitor eulachon population trends and represent a >80% cost savings over mark-recapture, which could be further increased with automated water sampling, reduced replication, and focused temporal sampling. Due to its logistical ease and affordability, eDNA sampling can facilitate monitoring a larger number of rivers and in remote locations where mark-recapture is infeasible.
引用
收藏
页码:457 / 467
页数:11
相关论文
共 42 条
[1]  
[Anonymous], 1951, University of California Publications in Statistic
[2]   The ecology of environmental DNA and implications for conservation genetics [J].
Barnes, Matthew A. ;
Turner, Cameron R. .
CONSERVATION GENETICS, 2016, 17 (01) :1-17
[3]  
Betts M.F., 1994, Alaska Department of Fish and Game, V213, P1
[4]   Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus) [J].
Biggs, Jeremy ;
Ewald, Naomi ;
Valentini, Alice ;
Gaboriaud, Coline ;
Dejean, Tony ;
Griffiths, Richard A. ;
Foster, Jim ;
Wilkinson, John W. ;
Arnell, Andy ;
Brotherton, Peter ;
Williams, Penny ;
Dunn, Francesca .
BIOLOGICAL CONSERVATION, 2015, 183 :19-28
[5]   Global climate change and potential effects on Pacific salmonids in freshwater ecosystems of southeast Alaska [J].
Bryant, M. D. .
CLIMATIC CHANGE, 2009, 95 (1-2) :169-193
[6]   Life history and age at maturity of an anadromous smelt, the eulachon Thaleichthys pacificus (Richardson) [J].
Clarke, A. D. ;
Lewis, A. ;
Telmer, K. H. ;
Shrimpton, J. M. .
JOURNAL OF FISH BIOLOGY, 2007, 71 (05) :1479-1493
[7]   The struggle to govern the commons [J].
Dietz, T ;
Ostrom, E ;
Stern, PC .
SCIENCE, 2003, 302 (5652) :1907-1912
[8]   Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys [J].
Doi, Hideyuki ;
Uchii, Kimiko ;
Takahara, Teruhiko ;
Matsuhashi, Saeko ;
Yamanaka, Hiroki ;
Minamoto, Toshifumi .
PLOS ONE, 2015, 10 (03)
[9]   Developing harvest strategies for low-value and data-poor fisheries: Case studies from three Australian fisheries [J].
Dowling, Natalie A. ;
Smith, David C. ;
Knuckey, Ian ;
Smith, Anthony D. M. ;
Domaschenz, Phil ;
Patterson, Heather M. ;
Whitelaw, Wade .
FISHERIES RESEARCH, 2008, 94 (03) :380-390
[10]   Species detection using environmental DNA from water samples [J].
Ficetola, Gentile Francesco ;
Miaud, Claude ;
Pompanon, Francois ;
Taberlet, Pierre .
BIOLOGY LETTERS, 2008, 4 (04) :423-425