Expression of human activin C protein in insect larvae infected with a recombinant baculovirus

被引：8

作者：

Kron, R ^{[1
]}

Schneider, C ^{[1
]}

Hötten, G ^{[1
]}

Bechtold, R ^{[1
]}

Pohl, J ^{[1
]}

机构：

[1] Biopharm GmbH, D-69115 Heidelberg, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 72卷 / 01期

关键词：

expression; baculovirus; activin; recombination;

D O I：

10.1016/S0166-0934(97)00215-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to generate dimeric recombinant transforming growth factor-beta (TGF-beta) proteins, expensive eucaryotic cell systems, such as CHO cells, are usually used. An alternative represents the expression of such proteins in insects using a baculovirus expression system. In this study, recombinant human activin C protein was expressed in Noctuidae larvae. On SDS-PAGE, the expressed protein has a size of about 15 kD under reducing conditions and of about 20 kD under non-reducing conditions. This suggests that activin C is expressed as a dimer and disulfide bridges can be formed. Compared with expression in eucaryotic cell culture systems, expression in insect larvae presents a rapid and low cost method, without the need for expensive tissue culture scale-ups or special equipment. (C) 1998 Elsevier Science B.V. All rights reserved.

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页码：9 / 14

页数：6

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