Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome

被引：4

作者：

Qiu, Xiaofen ^{[1
,2
,3
]}

Yu, Haiyan ^{[1
]}

Wu, Hongwei ^{[1
]}

Hu, Zhiyang ^{[1
]}

Zhou, Jun ^{[1
]}

Lin, Hua ^{[2
]}

Xue, Wen ^{[2
]}

Cai, Wanxia ^{[1
]}

Chen, Jiejing ^{[2
]}

Yan, Qiang ^{[2
]}

Dai, Weier ^{[4
]}

Yang, Ming ^{[2
]}

Tang, Donge ^{[1
]}

Dai, Yong ^{[1
,2
]}

机构：

[1] Jinan Univ, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Dept Clin Med Res Ctr,Shenzhen Peoples Hosp, Affiliated Hosp 1,Southern Univ Sci & Technol,Sec, Shenzhen 518020, Guangdong, Peoples R China

[2] 924 Hosp, Guangxi Key Lab Metab Dis Res, Dept Clin Lab Guilin, Guilin 541002, Guangxi, Peoples R China

[3] Guangxi Normal Univ, Coll Life Sci, Guilin 541004, Guangxi, Peoples R China

[4] Univ Texas Austin, Coll Nat Sci, Austin, TX 78712 USA

来源：

HUMAN GENOMICS | 2021年 / 15卷 / 01期

关键词：

Trisomy; 18; syndrome; single-cell sequencing; Transcription factors; Aneuploidy; Developmental regulation; PERIPHERAL-BLOOD; ORIGIN; STEM;

D O I：

10.1186/s40246-021-00338-z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background Trisomy 18 syndrome (Edwards syndrome, ES) is a type of aneuploidy caused by the presence of an extra chromosome 18. Aneuploidy is the leading cause of early pregnancy loss, intellectual disability, and multiple congenital anomalies. The research of trisomy 18 is progressing slowly, and the molecular characteristics of the disease mechanism and phenotype are still largely unclear. Results In this study, we used the commercial Chromium platform (10x Genomics) to perform sc-ATAC-seq to measure chromatin accessibility in 11,611 single umbilical cord blood cells derived from one trisomy 18 syndrome patient and one healthy donor. We obtained 13 distinct major clusters of cells and identified them as 6 human umbilical cord blood mononuclear cell types using analysis tool. Compared with the NC group, the ES group had a lower ratio of T cells to NK cells, the ratio of monocytes/DC cell population did not change significantly, and the ratio of B cell nuclear progenitor and megakaryocyte erythroid cells was higher. The differential genes of ME-0 are enriched in Human T cell leukemia virus 1 infection pathway, and the differential peak genes of ME-1 are enriched in apopotosis pathway. We found that CCNB2 and MCM3 may be vital to the development of trisomy 18. CCNB2 and MCM3, which have been reported to be essential components of the cell cycle and chromatin. Conclusions We have identified 6 cell populations in cord blood. Disorder in megakaryocyte erythroid cells implicates trisomy 18 in perturbing fetal hematopoiesis. We identified a pathway in which the master differential regulatory pathway in the ME-0 cell population involves human T cell leukemia virus 1 infection, a pathway that is dysregulated in patients with trisomy 18 and which may increase the risk of leukemia in patients with trisomy 18. CCNB2 and MCM3 in progenitor may be vital to the development of trisomy 18. CCNB2 and MCM3, which have been reported to be essential components of the cell cycle and chromatin, may be related to chromosomal abnormalities in trisomy 18.

引用

页数：13