Plasma N-glycome composition associates with chronic low back pain

被引：21

作者：

Trbojevic-Akmacic, Irena ^{[1
]}

Vuckovic, Frano ^{[1
]}

Vilaj, Marija ^{[1
]}

Skelin, Andrea ^{[1
,11
]}

Karssen, Lennart C. ^{[2
]}

Kristic, Jasminka ^{[1
]}

Juric, Julija ^{[1
]}

Momcilovic, Ana ^{[1
,3
]}

Simunovic, Jelena ^{[1
]}

Mangino, Massimo ^{[4
,5
]}

De Gregori, Manuela ^{[6
,7
]}

Marchesini, Maurizio ^{[7
,8
]}

Dagostino, Concetta ^{[7
,9
]}

Stambuk, Jerko ^{[1
]}

Novokmet, Mislav ^{[1
]}

Rauck, Richard ^{[10
]}

Aulchenko, Yurii S. ^{[2
]}

Primorac, Dragan ^{[11
,12
,13
,14
,15
]}

Kapural, Leonardo ^{[10
]}

Buyse, Klaas ^{[16
,17
]}

Mesotten, Dieter ^{[16
,17
]}

Williams, Frances M. K. ^{[4
]}

van Zundert, Jan ^{[16
,17
]}

Allegri, Massimo ^{[7
,18
]}

Lauc, Gordan ^{[1
,19
]}

机构：

[1] Genos Glycosci Res Lab, Zagreb, Croatia

[2] PolyOmica, Het Vlaggeschip 61, NL-5237 PA sHertogenbosch, Netherlands

[3] Leiden Univ, Med Ctr, Glyc & Glycoprote Grp, Ctr Prote & Metabol, Leiden, Netherlands

[4] Kings Coll London, Dept Twin Res & Genet Epidemiol, London, England

[5] Guys & St Thomas Fdn Trust, NIHR Biomed Res Ctr, London SE1 9RT, England

[6] Fdn IRCCS Policlin San Matteo, Pain Therapy Serv, Pavia, Italy

[7] SIMPAR Grp, Rome, Italy

[8] Parma Hosp, Anesthesia Intens Care & Pain Serv, Parma, Italy

[9] Univ Parma, Dept Med & Surg, Parma, Italy

[10] Carolinas Pain Inst, Winston Salem, NC USA

[11] St Catherine Specialty Hosp, Zabok Zagreb, Croatia

[12] JJ Strossmayer Univ Osijek, Sch Med, Osijek, Croatia

[13] Univ Split, Sch Med, Split, Croatia

[14] Penn State Univ, Eberly Coll Sci, University Pk, PA 16802 USA

[15] Childrens Hosp Srebrnjak, Zagreb, Croatia

[16] Ziekenhuis Oost Limburg, Dept Anesthesiol, Genk, Belgium

[17] Ziekenhuis Oost Limburg, Ctr Multidisciplinary Pain, Genk, Belgium

[18] IRCCS MultiMed Hosp, Anesthesia & Intens Care Serv, Milan, Italy

[19] Univ Zagreb, Fac Pharm & Biochem, A Kovacica 1, Zagreb 10000, Croatia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2018年 / 1862卷 / 10期

关键词：

Glycan biomarker; Low back pain; Plasma N-glycosylation; Retrospective study; ALPHA-1-ACID GLYCOPROTEIN; SACROILIAC JOINT; GLYCOSYLATION; GLYCANS; DISEASE; DIAGNOSIS; FEATURES; BINDING; TOOL;

D O I：

10.1016/j.bbagen.2018.07.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging. Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors. LBP may be characterized by an underlying inflammatory process. Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation. We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease. Methods: Plasma samples of 1128 CLBP patients and 760 healthy controls were collected in clinical centers in Italy, Belgium and Croatia and used for N-glycosylation profiling by hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) after N-glycans release, fluorescent labeling and clean-up. Observed N-glycosylation profiles have been compared with a cohort of 126 patients with acute inflammation that underwent abdominal surgery. Results: We have found a statistically significant increase in the relative amount of high-branched (tri-antennary and tetra-antennary) N-glycan structures on CLBP patients' plasma glycoproteins compared to healthy controls. Furthermore, relative amounts of disialylated and trisialylated glycan structures were increased, while high-mannose and glycans containing bisecting N-acetylglucosamine decreased in CLBP. Conclusions: Observed changes in CLBP on the plasma N-glycome level are consistent with N-glycosylation changes usually seen in chronic inflammation. General significance: To our knowledge, this is a first large clinical study on CLBP patients and plasma N-glycome providing a new glycomics perspective on potential disease pathology.

引用

页码：2124 / 2133

页数：10