Two-photon excitation of quantum-dot-based fluorescence resonance energy transfer and its applications

被引：104
作者：
Clapp, Aaron R.
Pons, Thomas
Medintz, Igor L.
Delehanty, James B.
Melinger, Joseph S.
Tiefenbrunn, Theresa
Dawson, Philip E.
Fisher, Brent R.
O'Rourke, Brian
Mattoussi, Hedi
机构：
[1] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA
[2] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA
[3] USN, Res Lab, Div Elect Sci & Technol, Washington, DC 20375 USA
[4] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[5] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[6] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
[7] MIT, Dept Chem, Cambridge, MA 02139 USA
[8] Johns Hopkins Univ, Sch Med, Inst Mol Cardiobiol, Baltimore, MD 21205 USA
来源：
ADVANCED MATERIALS | 2007年 / 19卷 / 15期
关键词：
D O I：
10.1002/adma.200602036
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Nonradiative fluorescence resonance energy transfer (FRET) between a luminescent quantum dot (QD) donor and a proximal dye brought in close proximity of the QD surface via conjugation with a dye-labeled peptide (or a protein) is shown (see figure). The system is excited with near IR irradiation (well below the absorption band of the QD), via a fast two-photon process, which produces a FRET signal with very low background contribution due to a substantially reduced nonlinear direct excitation of the dye.
引用
收藏
页码：1921 / +
页数：7
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