DNA-binding and photocleavage studies of [Ru(phen)2(NMIP)]2+

被引：39
作者：
Tan, LF
Chao, H
Liu, YJ
Li, H
Sun, B
Ji, LN
机构：
[1] Sun Yat Sen Univ, Dept Chem, Guangzhou 510275, Peoples R China
[2] Sun Yat Sen Univ, Minist Educ, Key Lab Gene Engn, Guangzhou 510275, Peoples R China
[3] Nanjing Univ, State Key Lab Coordinat Chem, Nanjing 210093, Peoples R China
[4] So China Normal Univ, Dept Chem, Guangzhou 510000, Peoples R China
来源：
INORGANICA CHIMICA ACTA | 2005年 / 358卷 / 07期
基金：
中国国家自然科学基金;
关键词：
ruthenium(II) complex; polypyridyl ligand; DNA-binding; photocleavage;
D O I：
10.1016/j.ica.2004.10.030
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
A novel ligand 2'-(2"-nitro-3",4"-methylenedioxyphenyl)imidazo[4',5'-f][1,10]-phenanthroline (NMIP) and its complex [Ru(phen)(2)(NMIP)](2+) have been synthesized and characterized by mass spectroscopy, H-1 NMR and cyclic voltammetry. Binding of the complex with calf thymus DNA (CT DNA) has been investigated by spectroscopic methods, viscosity and electrophoresis measurements. The experimental results indicate that [Ru(phen)(2)(NMIP)](2+) binds to DNA via partial intercalative mode and the individual enantiomers of it bind to DNA in different rates. [Ru(phen)(2)(NMIP)](2+) has also been found to promote cleavage of plasmid pBR 322 DNA from the supercoiled Form I to the open circular Form 11 upon irradiation. (c) 2004 Published by Elsevier B.V.
引用
收藏
页码：2191 / 2198
页数：8
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