Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons

被引：67

作者：

Aubry, Fabien ^{[1
]}

Nougairede, Antoine ^{[1
]}

de Fabritus, Lauriane ^{[1
]}

Querat, Gilles ^{[1
]}

Gould, Ernest A. ^{[1
]}

de Lamballerie, Xavier ^{[1
]}

机构：

[1] Aix Marseille Univ, IRD French Inst Res Dev, EHESP French Sch Publ Hlth, EPV UMR D Emergence Pathol Virales 190, F-13385 Marseille, France

来源：

JOURNAL OF GENERAL VIROLOGY | 2014年 / 95卷

关键词：

BORNE ENCEPHALITIS-VIRUS; CDNA-CLONES; GENOME; CONSTRUCTION; TRANSCRIPTS; MUTATION; STRAIN; DNA;

D O I：

10.1099/vir.0.068023-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.

引用

页码：2462 / 2467

页数：6

共 20 条

[1] Complete Genome of a Genotype I Japanese Encephalitis Virus Isolated from a Patient with Encephalitis in Vientiane, Lao PDR [J].

Aubry, Fabien ;

Vongsouvath, Manivanh ;

Nougairede, Antoine ;

Phetsouvanh, Rattanaphone ;

Sibounheuang, Bountoy ;

Charrel, Remi ;

Rattanavong, Sayaphet ;

Phommasone, Koukeo ;

Sengvilaipraserth, Onanong ;

de Lamballerie, Xavier ;

Newton, Paul N. ;

Dubot-Peres, Audrey .

GENOME ANNOUNCEMENTS, 2013, 1 (01)

[2]

Bacher JM, 2002, GENOME BIOL, V3

[3] A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication [J].

Bredenbeek, PJ ;

Kooi, EA ;

Lindenbach, B ;

Huijkman, N ;

Rice, CM ;

Spaan, WJM .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :1261-1268

[4] Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs [J].

Csoergo, Balint ;

Feher, Tamas ;

Timar, Edit ;

Blattner, Frederick R. ;

Posfai, Gyoergy .

MICROBIAL CELL FACTORIES, 2012, 11

[5] A Novel Bacterium-Free Method for Generation of Flavivirus Infectious DNA by Circular Polymerase Extension Reaction Allows Accurate Recapitulation of Viral Heterogeneity [J].

Edmonds, Judith ;

van Grinsven, Erinke ;

Prow, Natalie ;

Bosco-Lauth, Angela ;

Brault, Aaron C. ;

Bowen, Richard A. ;

Hall, Roy A. ;

Khromykh, Alexander A. .

JOURNAL OF VIROLOGY, 2013, 87 (04) :2367-2372

[6] Attenuation of Chikungunya Virus Vaccine Strain 181/Clone 25 Is Determined by Two Amino Acid Substitutions in the E2 Envelope Glycoprotein [J].

Gorchakov, Rodion ;

Wang, Eryu ;

Leal, Grace ;

Forrester, Naomi L. ;

Plante, Kenneth ;

Rossi, Shannan L. ;

Partidos, Charalambos D. ;

Adams, A. Paige ;

Seymour, Robert L. ;

Weger, James ;

Borland, Erin M. ;

Sherman, Michael B. ;

Powers, Ann M. ;

Osorio, Jorge E. ;

Weaver, Scott C. .

JOURNAL OF VIROLOGY, 2012, 86 (11) :6084-6096

[7] Development and analysis of a tick-borne encephalitis virus infectious clone using a novel and rapid strategy [J].

Gritsun, TS ;

Gould, EA .

JOURNAL OF VIROLOGICAL METHODS, 1998, 76 (1-2) :109-120

[8] Infectious transcripts of tick-borne encephalitis virus, generated in days by RT-PCR [J].

Gritsun, TS ;

Gould, EA .

VIROLOGY, 1995, 214 (02) :611-618

[9] DENGUE VIRUS-SPECIFIC AND FLAVIVIRUS GROUP DETERMINANTS IDENTIFIED WITH MONOCLONAL-ANTIBODIES BY INDIRECT IMMUNOFLUORESCENCE [J].

HENCHAL, EA ;

GENTRY, MK ;

MCCOWN, JM ;

BRANDT, WE .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1982, 31 (04) :830-836

[10] In planta engineering of viral RNA replicons:: Efficient assembly by recombination of DNA modules delivered by Agrobacterium [J].

Marillonnet, S ;

Giritch, A ;

Gils, M ;

Kandzia, R ;

Klimyuk, V ;

Gleba, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (18) :6852-6857

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