共 91 条
Local resources of polyribosomes and SER promote synapse enlargement and spine clustering after long-term potentiation in adult rat hippocampus
被引:52
作者:
Chirillo, Michael A.
[1
,2
]
Waters, Mikayla S.
[1
,3
]
Lindsey, Laurence F.
[1
,4
]
Bourne, Jennifer N.
[1
,5
]
Harris, Kristen M.
[1
]
机构:
[1] Univ Texas Austin, Dept Neurosci, Ctr Learning & Memory, Austin, TX 78712 USA
[2] Univ Belgrade, Fulbright US Scholar Program, Studentski Trg 1, Belgrade 11000, Serbia
[3] McGovern Med Sch Houston, 6431 Fannin St, Houston, TX 77030 USA
[4] Google Seattle, Seattle, WA 98103 USA
[5] Univ Colorado, Dept Cell & Dev Biol, Anschutz Med Campus, Aurora, CO 80045 USA
基金:
美国国家科学基金会;
关键词:
ENDOPLASMIC-RETICULUM;
DENDRITIC SPINES;
SECRETORY TRAFFICKING;
INTEGRAL MEMBRANE;
INPUT SPECIFICITY;
CALCIUM-RELEASE;
DIFFERENT FORMS;
CA1;
DENDRITES;
MICE LACKING;
SYNAPTOPODIN;
D O I:
10.1038/s41598-019-40520-x
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Synapse clustering facilitates circuit integration, learning, and memory. Long-term potentiation (LTP) of mature neurons produces synapse enlargement balanced by fewer spines, raising the question of how clusters form despite this homeostatic regulation of total synaptic weight. Three-dimensional reconstruction from serial section electron microscopy (3DEM) revealed the shapes and distributions of smooth endoplasmic reticulum (SER) and polyribosomes, subcellular resources important for synapse enlargement and spine outgrowth. Compared to control stimulation, synapses were enlarged two hours after LTP on resource-rich spines containing polyribosomes (4% larger than control) or SER (15% larger). SER in spines shifted from a single tubule to complex spine apparatus after LTP. Negligible synapse enlargement (0.6%) occurred on resource-poor spines lacking SER and polyribosomes. Dendrites were divided into discrete synaptic clusters surrounded by asynaptic segments. Spine density was lowest in clusters having only resource-poor spines, especially following LTP. In contrast, resource-rich spines preserved neighboring resource-poor spines and formed larger clusters with elevated total synaptic weight following LTP. These clusters also had more shaft SER branches, which could sequester cargo locally to support synapse growth and spinogenesis. Thus, resources appear to be redistributed to synaptic clusters with LTP-related synapse enlargement while homeostatic regulation suppressed spine outgrowth in resource-poor synaptic clusters.
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页数:14
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