Autologous Stem Cell Transplantation: Leukapheresis Product has Anti-angiogenic Effects In Vivo Correlating with Neutrophil-derived VEGFR1

被引：0

作者：

Luethy, Anita ^{[1
,2
]}

Stenner, Frank ^{[3
]}

Lohri, Corinne ^{[3
]}

Muller, Christoph ^{[1
,2
]}

Samaras, Panagiotis ^{[3
]}

Steiner, Rudolf ^{[3
]}

Van den Broek, Maries ^{[3
]}

Mischo, Axel ^{[3
]}

Renner, Christoph ^{[3
]}

Knuth, Alexander ^{[3
]}

Ruegg, Curzio ^{[4
]}

Wenger, Roland H. ^{[1
,2
]}

Zweifel, Martin ^{[3
]}

机构：

[1] Univ Zurich, Inst Physiol, Zurich, Switzerland

[2] Univ Zurich, Zurich Ctr Integrat Human Physiol ZIHP, Zurich, Switzerland

[3] Univ Zurich Hosp, Dept Oncol, CH-8091 Zurich, Switzerland

[4] Univ Fribourg, Fac Sci, Dept Med, CH-1700 Fribourg, Switzerland

来源：

ANTICANCER RESEARCH | 2011年 / 31卷 / 10期

关键词：

Alternative splicing; angiogenesis; autologous stem cell transplantation; chick chorioallantoic membrane assay; high-dose chemotherapy; placental growth factor; vascular endothelial growth factor; VEGFR1; ENDOTHELIAL GROWTH-FACTOR; BONE-MARROW-TRANSPLANTATION; ACUTE MYOCARDIAL-INFARCTION; PERIPHERAL-BLOOD; PROGENITOR CELLS; MULTIPLE-MYELOMA; FACTOR RECEPTOR-1; TYROSINE KINASE; LIMB ISCHEMIA; THERAPEUTIC ANGIOGENESIS;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: High-dose chemotherapy (HDC) followed by autologous stem cell transplantation (ASCT) is used for the treatment of hemato-oncologic malignancies. In this study, we measured the effect of HDC/ASCT on plasma concentrations of antiangiogenic soluble vascular endothelial growth factor receptor I (sVEGFR1) and of leukapheresis products (LP) and patient serum on chick chorioallantoic (CAM) angiogenesis. Materials and Methods: VEGFR1- and CD34-expressing cells of leukapheresis products were analyzed by flow cytometry. Alternatively spliced isoforms of VEGFR1 mRNA were quantified using reverse transcription PCR. Results: Plasma concentrations of sVEGFR1 decreased after HDC, but significantly increased after ASCT. In the CAM assay, sera of patients elicited a proangiogenic effect before and after HDC, but a strong antiangiogenic response after ASCT, comparable to that of bevacizumab at therapeutic concentrations. LP contains high concentrations of sVEGFR1, and high density of VEGFR1(+) neutrophilic granulocytes, in which mRNA expression is shifted toward the soluble VEGFR1 isoform. Conclusion: Neutrophil-derived antiangiogenic sVEGFR1 within the LP may contribute to the therapeutic efficacy of ASCT.

引用

页码：3115 / 3124

页数：10

共 1 条

[1] Synergistic Anti-Angiogenic Effects Using Peptide-Based Combinatorial Delivery of siRNAs Targeting VEGFA, VEGFR1, and Endoglin Genes
Egorova, Anna A.
Shtykalova, Sofia, V
Maretina, Marianna A.
Sokolov, Dmitry, I
Selkov, Sergei A.
Baranov, Vladislav S.
Kiselev, Anton, V
PHARMACEUTICS, 2019, 11 (06)

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