Identical subunit topographies of human and yeast 20S proteasomes

被引:18
|
作者
Dahlmann, B
Kopp, F
Kristensen, P
Hendil, KB
机构
[1] Diabet Forschungsinst, D-40225 Dusseldorf, Germany
[2] Univ Copenhagen, August Krogh Inst, DK-2100 Copenhagen, Denmark
关键词
human proteasome; quaternary structure; epitope mapping; immunoelectron microscopy; subunit arrangement;
D O I
10.1006/abbi.1999.1104
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The arrangement of subunits in human 20S proteasomes was recently determined by us by immunoelectron microscopy and chemical cross-linking. The positions of 4 of the 14 subunits differed from those found in the yeast proteasome by X-ray crystallography. Double labeling of human 20S proteasomes with antibodies to subunits C2 and C5 has now shown that these subunits are nearest neighbors. The result contradicts our published model for the human proteasome but is in accordance with the subunit arrangement in yeast proteasomes, suggesting that yeast and human proteasomes most probably have identical subunit arrangements. Immunoelectron microscopy also showed that the C-terminal extension at the human C2 subunit is flexible but takes up a well-defined position in the proteasome. (C) 1999 Academic Press.
引用
收藏
页码:296 / 300
页数:5
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