Discovery of Cationic Polymers for Non-Viral Gene Delivery Using Combinatorial Approaches

被引：62

作者：

Barua, Sutapa ^{[1
]}

Ramos, James ^{[1
]}

Potta, Thrimoorthy ^{[1
]}

Taylor, David ^{[1
]}

Huang, Huang-Chiao ^{[1
]}

Montanez, Gabriela ^{[1
]}

Rege, Kaushal ^{[1
]}

机构：

[1] Arizona State Univ, Tempe, AZ 85287 USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2011年 / 14卷 / 10期

基金：

美国国家科学基金会;

关键词：

Parallel screening; polymer library; transfection; gene delivery; transgene expression; cytotoxicity; REGIONAL LYMPH-NODES; IN-VITRO; PARALLEL SYNTHESIS; PLASMID DNA; TRANSFECTION EFFICIENCY; VIRAL VECTORS; SMALL LIBRARY; CHITOSAN; POLYETHYLENIMINE; NANOPARTICLES;

D O I：

10.2174/138620711797537076

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene therapy is an attractive treatment option for diseases of genetic origin, including several cancers and cardiovascular diseases. While viruses are effective vectors for delivering exogenous genes to cells, concerns related to insertional mutagenesis, immunogenicity, lack of tropism, decay and high production costs necessitate the discovery of non-viral methods. Significant efforts have been focused on cationic polymers as non-viral alternatives for gene delivery. Recent studies have employed combinatorial syntheses and parallel screening methods for enhancing the efficacy of gene delivery, biocompatibility of the delivery vehicle, and overcoming cellular level barriers as they relate to polymer-mediated transgene uptake, transport, transcription, and expression. This review summarizes and discusses recent advances in combinatorial syntheses and parallel screening of cationic polymer libraries for the discovery of efficient and safe gene delivery systems.

引用

页码：908 / 924

页数：17

共 96 条

[11]

Boulikas T, 1997, ANTICANCER RES, V17, P1471

[12] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].