P-site tRNA is a crucial initiator of ribosomal frameshifting

被引：69

作者：

Baranov, PV ^{[1
]}

Gesteland, RF ^{[1
]}

Atkins, JF ^{[1
]}

机构：

[1] Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA

来源：

RNA | 2004年 / 10卷 / 02期

关键词：

translation; recoding; kinetic model; frameshifting; ribosome;

D O I：

10.1261/rna.5122604

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of some genes requires a high proportion of ribosomes to shift at a specific site into one of the two alternative frames. This utilized frameshifting provides a unique tool for studying reading frame control. Peptidyl-tRNA slippage has been invoked to explain many cases of programmed frameshifting. The present work extends this to other cases. When the A-site is unoccupied, the P-site tRNA can be repositioned forward with respect to mRNA (although repositioning in the minus direction is also possible). A kinetic model is presented for the influence of both, the cognate tRNAs competing for overlapping codons in A-site, and the stabilities of P-site tRNA:mRNA complexes in the initial and new frames. When the A-site is occupied, the P-site tRNA can be repositioned backward. Whether frameshifting will happen depends on the ability of the A-site tRNA to subsequently be repositioned to maintain physical proximity of the tRNAs. This model offers an alternative explanation to previously published mechanisms of programmed frameshifting, such as out-of-frame tRNA binding, and a different perspective on simultaneous tandem tRNA slippage.

引用

页码：221 / 230

页数：10

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