Optically triggered immune response through photocaged oligonucleotides

被引：21
|
作者：
Govan, Jeane M. [1 ]
Young, Douglas D. [1 ]
Lively, Mark O. [2 ]
Deiters, Alexander [1 ,3 ]
机构：
[1] N Carolina State Univ, Raleigh, NC 27167 USA
[2] Wake Forest Univ, Bowman Gray Sch Med, Winston Salem, NC 27157 USA
[3] Univ Pittsburgh, Pittsburgh, PA 15260 USA
来源：
TETRAHEDRON LETTERS | 2015年 / 56卷 / 23期
关键词：
Oligonucleotide synthesis; Light-activation; Photocaged nucleotides; Immune response; Toll-like receptors; PHOTOCHEMICAL CONTROL; OPTOCHEMICAL CONTROL; LIGHT-ACTIVATION; MAMMALIAN-CELLS; BACTERIAL-DNA; CPG-DNA; VACCINE; IMMUNOGENICITY;
D O I：
10.1016/j.tetlet.2015.01.165
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Bacterial and viral CpG oligonculeotides are unmethylated cytosine-phosphate-guanosine dinucleotide sequences and trigger an innate immune response through activation of the toll-like receptor 9 (TLR9). We have developed synthetic photocaged CpGs via site-specific incorporation of nitropiperonyloxymethyl (NPOM)-caged thymidine residues. These oligonucleotides enable the optical control of TLR9 function and thereby provide light-activation of an immune response. We provide a proof-of-concept model by applying a reporter assay in live cells and by quantification of endogenous production of interleukin 6. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3639 / 3642
页数：4
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