A Synthetic Hydrazone Derivative Acts as an Apoptotic Inducer with Chemopreventive Activity on a Tongue Cancer Cell Line

被引:0
|
作者
Zulkepli, Nur Ayunie [1 ]
Rou, Karen Voon Kai [1 ]
Sulaiman, Wan Nur Hidayati Wan [1 ]
Salhin, Abdussalam [2 ]
Saad, Bahruddin [2 ]
Seeni, Azman [1 ,3 ]
机构
[1] Univ Sains Malaysia, Sch Dent Sci, George Town, Malaysia
[2] Univ Sains Malaysia, Sch Chem Sci, George Town, Malaysia
[3] Univ Sains Malaysia, Adv Med & Dent Inst, George Town, Malaysia
关键词
Chemoprevention; apoptosis; hydrazones; tongue cancer cells; PRIMARY CYTOTOXICITY EVALUATION;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
One of the main aims of cancer chemopreventive studies is to identify ideal apoptotic inducers, especially examples which can induce early apoptotic activity. The present investigation focused on chemopreventive effects of a hydrazone derivative using an in vitro model with tongue cancer cells. Alteration in cell morphology was ascertained, along with stage in the cell cycle and proliferation, while living-dead status of the cells was confirmed under a confocal microscope. In addition, cytotoxicity test was performed using normal mouse skin fibroblast cells. The results showed that the compound inhibited the growth of tongue cancer cells with an inhibitory concentration (IC(50)) of 0.01 mg/ml in a dose and time-dependent manner, with a two-fold increase in early apoptotic activity and G0G1 phase cell cycle arrest compared to untreated cells. Exposure to the compound also resulted in alterations of cell morphology including vacuolization and cellular shrinkage. Confocal microscope analysis using calcein and ethidium staining confirmed that the compound caused cell death, whereas no cytotoxic effects on normal mouse skin fibroblast cells were observed. In conclusion, the findings in this study suggested that the hydrazone derivative acts as an apoptotic inducer with anti-proliferative chemopreventive activity in tongue cancer cells.
引用
收藏
页码:259 / 263
页数:5
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