Improved designs for pET expression plasmids increase protein production yield in Escherichia coli

被引：115

作者：

Shilling, Patrick J. ^{[1
]}

Mirzadeh, Kiavash ^{[1
,2
]}

Cumming, Alister J. ^{[1
]}

Widesheim, Magnus ^{[1
]}

Kock, Zoe ^{[1
,3
]}

Daley, Daniel O. ^{[1
]}

机构：

[1] Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden

[2] Xbrane Biopharma, Solna, Sweden

[3] Goethe Univ, Frankfurt, Germany

来源：

COMMUNICATIONS BIOLOGY | 2020年 / 3卷 / 01期

基金：

瑞典研究理事会;

关键词：

RIBOSOME BINDING-SITES; T7; RNA-POLYMERASE; TRANSLATION INITIATION; SEQUENCE; CODON; MTH1; DNA;

D O I：

10.1038/s42003-020-0939-8

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The pET series of expression plasmids are widely used for recombinant protein production in Escherichia coli. The genetic modules controlling transcription and translation in these plasmids were first described in the 1980s and have not changed since. Herein we report design flaws in these genetic modules. We present improved designs and demonstrate that, when incorporated into pET28a, they support increases in protein production. The improved designs are applicable to most of the 103 vectors in the pET series and can be easily implemented. Patrick Shilling et al. increase the protein production yield from the pET28a expression plasmid by modifying the genetic modules that control transcription and translation initiation. These improved designs are applicable to most vectors in the pET series and can be easily implemented.

引用

页数：8

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