Quinones in plant plasma membranes -: a missing link?

被引:41
作者
Lüthje, S
Van Gestelen, P
Córdoba-Pedregosa, MC
González-Reyes, JA
Asard, H
Villalba, JM
Böttger, M
机构
[1] Univ Hamburg, Inst Allgemeine Bot, D-22609 Hamburg, Germany
[2] Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium
[3] Natl Univ Cordoba, Fac Ciencias, Dept Biol Celular, Cordoba, Spain
关键词
NAD(P)H dehydrogenase; b-type cytochrome; flavin; naphthoquinones; plasma membrane; Zea mays L;
D O I
10.1007/BF01279292
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The occurrence of a vitamin-K-like substance (naphthoquinone group) and flavins (flavin mononucleotide and flavin adenine dinucleotide) is demonstrated in plasma membranes isolated from maize (Zea mays L.) roots, on the basis of high-pressure liquid chromotography and spectral analysis. At least three NAD(P)H dehydrogenases could be purified to homogeneity from this plant material, Two of these proteins (25 and 30 kDa) reduce hexacyanoferrate III and quinones, while the third (41 kDa) reduces oxalacetic acid but not hexacyanoferrate III in the presence of NADH. Low-temperature spectra demonstrate the occurrence of a h-type cytochrome in plasma membranes isolated from maize roots. The latter compound could be reduced by ascorbic acid (E-0' > +80 mV) and shows an alpha-band maximum at 559 nm (at -196 degrees C). NADH-dependent cytochrome b reduction could be observed only in the presence of detergent and increased after preincubation with vitamin K-3 (menadione). On the basis of the presented data a possible function of naphthoquinones in plasma membrane electron transfer is discussed.
引用
收藏
页码:43 / 51
页数:9
相关论文
共 35 条
[1]   DUROQUINONE-STIMULATED NADH OXIDASE AND B-TYPE CYTOCHROMES IN THE PLASMA-MEMBRANE OF CAULIFLOWER AND MUNG BEANS [J].
ASARD, H ;
CAUBERGS, R ;
RENDERS, D ;
DEGREEF, JA .
PLANT SCIENCE, 1987, 53 (02) :109-119
[2]   TRANSMEMBRANE ELECTRON-TRANSPORT IN ASCORBATE-LOADED PLASMA-MEMBRANE VESICLES FROM HIGHER-PLANTS INVOLVES A B-TYPE CYTOCHROME [J].
ASARD, H ;
HOREMANS, N ;
CAUBERGS, RJ .
FEBS LETTERS, 1992, 306 (2-3) :143-146
[3]  
ASARD H, 1994, BELG J BOT, V127, P171
[4]   CYTOCHROMES OF PLANT PLASMA-MEMBRANES - CHARACTERIZATION BY ABSORBANCE DIFFERENCE SPECTROPHOTOMETRY AND REDOX TITRATION [J].
ASKERLUND, P ;
LARSSON, C ;
WIDELL, S .
PHYSIOLOGIA PLANTARUM, 1989, 76 (02) :123-134
[5]  
BARR R, 1992, BIOCHEM INT, V27, P449
[6]   ON THE PRESENCE OF INSIDE-OUT PLASMA-MEMBRANE VESICLES AND VANADATE-INHIBITED K+, MG2+-ATPASE IN MICROSOMAL FRACTIONS FROM WHEAT AND MAIZE ROOTS [J].
BERCZI, A ;
LARSSON, C ;
WIDELL, S ;
MOLLER, IM .
PHYSIOLOGIA PLANTARUM, 1989, 77 (01) :12-19
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   TOPOGRAPHY OF THE 27-AND 31-KDA ELECTRON-TRANSPORT PROTEINS IN THE ONION ROOT PLASMA-MEMBRANE [J].
CORDOBA, MC ;
SERRANO, A ;
CORDOBA, F ;
GONZALEZREYES, JA ;
NAVAS, P ;
VILLALBA, JM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 216 (03) :1054-1059
[9]  
CRAMER WA, 1991, CURR TOP BIOENERG, V16, P179
[10]  
Crane F, 1993, CLIN INVEST S, V71, P55