MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae

被引：74

作者：

Zhong, Kaili ^{[1
,2
]}

Li, Xiao ^{[1
,2
]}

Le, Xinyi ^{[1
,2
]}

Kong, Xiangyi ^{[1
,2
]}

Zhang, Haifeng ^{[1
,2
]}

Zheng, Xiaobo ^{[1
,2
]}

Wang, Ping ^{[3
]}

Zhang, Zhengguang ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing, Jiangsu, Peoples R China

[2] Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing, Peoples R China

[3] Louisiana State Univ, Dept Pediat, Hlth Sci Ctr, New Orleans, LA USA

来源：

PLOS PATHOGENS | 2016年 / 12卷 / 08期

基金：

美国国家科学基金会;

关键词：

WD REPEAT PROTEIN; ASEXUAL DIFFERENTIATION; TRANSCRIPTION FACTOR; TURGOR GENERATION; PLANT INFECTION; FULL VIRULENCE; AUTOPHAGY; DNM1P; GTPASE; GRISEA;

D O I：

10.1371/journal.ppat.1005823

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dynamins are large superfamily GTPase proteins that are involved in various cellular processes including budding of transport vesicles, division of organelles, cytokinesis, and pathogen resistance. Here, we characterized several dynamin-related proteins from the rice blast fungus Magnaporthe oryzae and found that MoDnm1 is required for normal functions, including vegetative growth, conidiogenesis, and full pathogenicity. In addition, we found that MoDnm1 co-localizes with peroxisomes and mitochondria, which is consistent with the conserved role of dynamin proteins. Importantly, MoDnm1-dependent peroxisomal and mitochondrial fission involves functions of mitochondrial fission protein MoFis1 and WD-40 repeat protein MoMdv1. These two proteins display similar cellular functions and subcellular localizations as MoDnm1, and are also required for full pathogenicity. Further studies showed that MoDnm1, MoFis1 and MoMdv1 are in complex to regulate not only peroxisomal and mitochondrial fission, pexophagy and mitophagy progression, but also appressorium function and host penetration. In summary, our studies provide new insights into how MoDnm1 interacts with its partner proteins to mediate peroxisomal and mitochondrial functions and how such regulatory events may link to differentiation and pathogenicity in the rice blast fungus.

引用

页数：34