Characterization of spiny lobsters from Bangladesh waters using morphology, COI and 16S rRNA sequences

被引：4

作者：

Ahmed, Md. Sagir ^{[1
]}

Barua, Anindita ^{[2
]}

Datta, Sujan Kumar ^{[1
]}

Saha, Tonmoy ^{[3
]}

Antu, Durjoy Raha ^{[3
]}

Ahmed, Sumaiya ^{[3
]}

机构：

[1] Univ Dhaka, Dept Zool, Dhaka 1000, Bangladesh

[2] Univ Dhaka, Dept Genet Engn & Biotechnol, Dhaka 1000, Bangladesh

[3] Jagannath Univ, Dept Zool, Dhaka 1100, Bangladesh

来源：

HELIYON | 2022年 / 8卷 / 02期

关键词：

Spiny lobster; COI; 16S rRNA; DNA Barcoding; Bangladesh; CRUSTACEA-DECAPODA-SCYLLARIDAE; SLIPPER LOBSTER; MITOCHONDRIAL; PANULIRUS; COAST;

D O I：

10.1016/j.heliyon.2022.e08846

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study aims to taxonomically identify and characterise the phylogenetic relationships of spiny lobsters based on mitochondrial cytochrome c oxidase I (COI) and 16S rRNA genes from Bangladesh waters. A total of 19 barcode sequences (10 partial COI sequences and 9 partial 16S rRNA) were successfully generated from 12 collected spiny lobster samples representing four species belonging to the family Palinuridae. The average genetic distances within and between species were 0.834 +/- 0.427 and 17.810 +/- 0.830, respectively, in COI and 0.107 +/- 0.255 and 8.401 +/- 2.547, respectively, in 16S rRNA genes. The successful amplification rate of 16S rRNA was higher than that of the COI marker. In the maximum likelihood (ML) tree, the sequences of the same species were clustered together under a single clade for both COI and 16S rRNA, which supports the efficacy of both marker genes in differentiating lobster species.

引用

页数：9

共 31 条

[1]

[Anonymous], 1991, Herpetol. Rev.

[2]

Bangladesh Gazettes, 2012, WILDLIFE CONSERVATIO, P127

[3] A revision of the shovel-nosed lobsters of the genus Thenus (Crustacea: Decapoda: Scyllaridae), with descriptions of three new species [J].