Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

被引：28

作者：

Hesselink, AT

van den Brule, AJC

Groothuismink, ZMA

Molano, A

Berkhof, J

Meijer, CJLM

Snijders, PJF

机构：

[1] Vrije Univ Amsterdam, Dept Pathol, Med Ctr, NL-1081 HV Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Dept Biostat & Epidemiol, Med Ctr, NL-1081 HV Amsterdam, Netherlands

[3] Stichting PAMM, Pathol Lab, Eindhoven, Netherlands

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 09期

关键词：

D O I：

10.1128/JCM.43.9.4868-4871.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

引用

收藏

页码：4868 / 4871

页数：4

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