Improving Rice Blast Resistance by Mining Broad-Spectrum Resistance Genes at Pik Locus

被引：10

作者：

Zhou Ying ^{[1
]}

Wan Tao ^{[1
]}

Yuan Bin ^{[3
]}

Lei Fang ^{[4
]}

Chen Meijuan ^{[5
]}

Wang Qiong ^{[1
]}

Huang Ping ^{[6
]}

Kou Shuyan ^{[6
]}

Qiu Wenxiu ^{[1
]}

Liu Li ^{[2
]}

机构：

[1] Wuhan Univ Sci & Technol, Coll Life Sci & Hlth, Wuhan 430065, Peoples R China

[2] Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China

[3] Minist Agr & Rural Affairs, Key Lab Integrated Management Crops Cent China, Hubei Key Lab Crop Dis Insect Pests & Weeds Contr, Wuhan 430064, Peoples R China

[4] Huazhong Agr Univ, Coll Informat, Wuhan 430070, Peoples R China

[5] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

[6] Yunnan Acad Agr Sci, Inst Food Crops, Kunming 650205, Yunnan, Peoples R China

来源：

RICE SCIENCE | 2022年 / 29卷 / 02期

基金：

中国国家自然科学基金;

关键词：

genetic diversity; near-isogenic line; Pik gene; resistance gene allele; rice blast; R gene; REPEAT PROTEIN; ENCODES; DIVERSITY; CLONING; MEMBER; RACE;

D O I：

10.1016/j.rsci.2022.01.002

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Magnaporthe oryzae is known for its genetic diversity and pathogenic variability, leading to rapid breakdown of resistance in rice. Incorporating multiple broad-spectrum blast resistance genes into rice cultivars would extend disease resistance longevity. Effective resistance breeding in rice therefore requires continual enrichment of the reservoir of resistance genes and alleles. We conducted a large-scale screen of rice blast resistance in about 2 000 rice accessions. Among them, 247 accessions showed at least medium resistance to the natural infection of rice blast and 7 novel Pik alleles were identified from them. Variations in gene sequences were then correlated with the phenotypic trait to enable the identification of favorable alleles. Among the seven novel Pik alleles, the resistant rate of Pik-R0/ME/7017 donors was greater than 80%, and the disease score was less than 3. Through molecular marker-assisted backcross breeding, we successfully transferred the three Pik alleles, Pik-R0/ME/7017, into an elite cultivated line Kongyu 131 to obtain BC3F2 lines, which showed enhanced resistance to rice blast compared with the recurrent parent. Assessment of these near-isogenic lines in the greenhouse using 31 isolates of M. oryzae from Heilongjiang Province of China revealed that the resistant levels of the BC3F2 lines with Pik-R0/ME/7017 were significantly higher than those of the established cloned resistance genes Pik-m and Pi1. Exploring such alleles will enrich our gene library for resistance to rice blast.

引用

页码：133 / 142

页数：10

共 42 条

[21] The in silico map-based cloning of Pi36, a rice coiled-coil-nucleotide-binding site-leucine-rich repeat gene that confers race-specific resistance to the blast fungus [J].

Liu, Xinqiong ;

Lin, Fei ;

Wang, Ling ;

Pan, Qinghua .

GENETICS, 2007, 176 (04) :2541-2549

[22] Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice [J].

Mi, Jiaming ;

Yang, Dabing ;

Chen, Yi ;

Jiang, Jiefeng ;

Mou, Haipeng ;

Huang, Junbin ;

Ouyang, Yidan ;

Mou, Tongmin .

RICE, 2018, 11

[23] Blast resistance in rice: a review of conventional breeding to molecular approaches [J].

Miah, G. ;

Rafii, M. Y. ;

Ismail, M. R. ;

Puteh, A. B. ;

Rahim, H. A. ;

Asfaliza, R. ;

Latif, M. A. .

MOLECULAR BIOLOGY REPORTS, 2013, 40 (03) :2369-2388

[24] RAPID ISOLATION OF HIGH MOLECULAR-WEIGHT PLANT DNA [J].

MURRAY, MG ;

THOMPSON, WF .

NUCLEIC ACIDS RESEARCH, 1980, 8 (19) :4321-4325

[25] The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice [J].

Qu, SH ;

Liu, GF ;

Zhou, B ;

Bellizzi, M ;

Zeng, LR ;

Dai, LY ;

Han, B ;

Wang, GL .

GENETICS, 2006, 172 (03) :1901-1914

[26] High-resolution mapping, cloning and molecular characterization of the Pi-kh supercript stop gene of rice, which confers resistance to Magnaporthe grisea [J].

Sharma, TR ;

Madhav, MS ;

Singh, BK ;

Shanker, P ;

Jana, TK ;

Dalal, V ;

Pandit, A ;

Singh, A ;

Gaikwad, K ;

Upreti, HC ;

Singh, NK .

MOLECULAR GENETICS AND GENOMICS, 2005, 274 (06) :569-578

[27]

Shen M, 2004, RICE BLAST DIS, P321

[28] Against the grain: safeguarding rice from rice blast disease [J].

Skamnioti, Pari ;

Gurr, Sarah J. .

TRENDS IN BIOTECHNOLOGY, 2009, 27 (03) :141-150

[29] Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars [J].

Tian, Dagang ;

Chen, Zaijie ;

Chen, Ziqiang ;

Zhou, Yuanchang ;

Wang, Zonghua ;

Wang, Feng ;

Chen, Songbiao .

RICE, 2016, 9

[30] The arms race between Magnaporthe oryzae and rice: Diversity and interaction of Avr and R genes [J].

Wang Bao-hua ;

Ebbole, Daniel J. ;

Wang Zong-hua .

JOURNAL OF INTEGRATIVE AGRICULTURE, 2017, 16 (12) :2746-2760

← 1 2 3 4 5 →