Identification of the proteins associated with low potassium tolerance in cultivated and Tibetan wild barley

被引:22
作者
Zeng, Jianbin [1 ]
He, Xiaoyan [1 ]
Quan, Xiaoyan [1 ]
Cai, Shengguan [1 ]
Han, Yong [1 ]
Nadira, Umme Aktari [1 ]
Zhang, Guoping [1 ]
机构
[1] Zhejiang Univ, Dept Agron, Key Lab Crop Germplasm Resource Zhejiang Prov, Hangzhou 310058, Zhejiang, Peoples R China
关键词
Hordeum vulgare; Hordeum spontaneum; Ion homeostasis; Plant proteomics; Low potassium tolerance; PHENYLALANINE AMMONIA-LYASE; TRANSCRIPTOME ANALYSIS; ARABIDOPSIS-THALIANA; GENOTYPES DIFFER; MESSENGER-RNAS; DROUGHT STRESS; SALT TOLERANCE; RICE; EFFICIENCY; RESPONSES;
D O I
10.1016/j.jprot.2015.05.025
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In previous studies, we found Tibetan wild barley accessions with high tolerance to low K. In this study, ionomics and proteomics analyses were done on two wild genotypes (XZ153, tolerant and XZ141, sensitive), and a cultivar (B1031, tolerance to low K) to understand the mechanism of low-K tolerance. XZ153 was much less affected by low K stress than the other two genotypes in plant biomass and shoot K content. A total of 288 differentially accumulated proteins were identified between low-K and normal K treated plants. Among them, 129 proteins related to low-K tolerance were mainly involved in defense, transcription, signal transduction, energy, and protein synthesis. The analysis of tandem mass tag (TMT) detected 51 proteins which were increased in relative abundance under low K in XZ153, but unaltered or decreased in XZ141. The proteomics results showed that XZ153 is highly capable of rearranging ion homeostasis and developing an antioxidant defense system under low-K stress. Moreover, ethylene response and phenylpropanoid pathways could determine the genotypic difference in low-K tolerance. The current results confirmed the possibility of Tibetan wild barley providing low-K tolerant germplasm and identified some candidate proteins for use in developing the cultivars with low-K tolerance. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:1 / 11
页数:11
相关论文
共 60 条
[1]   The effect of potassium nutrition on pest and disease resistance in plants [J].
Amtmann, Anna ;
Troufflard, Stephanie ;
Armengaud, Patrick .
PHYSIOLOGIA PLANTARUM, 2008, 133 (04) :682-691
[2]  
[Anonymous], 2012, EURASIP J WIREL COMM, DOI DOI 10.1186/1687-1499-2012-1
[3]   Biotechnological approach of improving plant salt tolerance using antioxidants as markers [J].
Ashraf, M. .
BIOTECHNOLOGY ADVANCES, 2009, 27 (01) :84-93
[4]   Nutrient use efficiency in plants [J].
Baligar, VC ;
Fageria, NK ;
He, ZL .
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 2001, 32 (7-8) :921-950
[5]   The role of potassium in alleviating detrimental effects of abiotic stresses in plants [J].
Cakmak, I .
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, 2005, 168 (04) :521-530
[6]   Change in phenylalanine ammonia lyase activity and isozyme patterns of polyphenol oxidase and peroxidase by salicylic acid leading to enhance resistance in cowpea against Rhizoctonia solani [J].
Chandra, Amaresh ;
Saxena, Raghvendra ;
Dubey, Archana ;
Saxena, Pradeep .
ACTA PHYSIOLOGIAE PLANTARUM, 2007, 29 (04) :361-367
[7]   Canola genotypes differ in potassium efficiency during vegetative growth [J].
Damon, P. M. ;
Osborne, L. D. ;
Rengel, Z. .
EUPHYTICA, 2007, 156 (03) :387-397
[8]   Influence of potassium nitrate on antioxidant level and secondary metabolite genes under cold stress in Panax ginseng [J].
Devi, B. S. R. ;
Kim, Y. J. ;
Selvi, S. K. ;
Gayathri, S. ;
Altanzul, K. ;
Parvin, S. ;
Yang, D. U. ;
Lee, O. R. ;
Lee, S. ;
Yang, D. C. .
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 2012, 59 (03) :318-325
[9]   STRESS-INDUCED PHENYLPROPANOID METABOLISM [J].
DIXON, RA ;
PAIVA, NL .
PLANT CELL, 1995, 7 (07) :1085-1097
[10]   A role for SR proteins in plant stress responses [J].
Duque, Paula .
PLANT SIGNALING & BEHAVIOR, 2011, 6 (01) :49-54