Analysis of reboxetine, a novel antidepressant drug, in pharmaceutical tablets by capillary electrophoresis and derivative spectrophotometry

被引:14
作者
Raggi, MA
Bugamelli, F
Sabbioni, C
Ferranti, A
Fanali, S
Volterra, V
机构
[1] Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy
[2] CNR, Inst Chromatog, Area Ric Roma, I-00016 Monterotondo, Rome, Italy
[3] Univ Bologna, Psychiat Clin, I-40123 Bologna, Italy
关键词
reboxetine; derivative spectrophotometry; capillary electrophoresis; pharmaceutical formulations;
D O I
10.1016/S0731-7085(01)00540-4
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The recent antidepressant drug reboxetine was quantified in pharmaceutical tablets by derivative spectrophotometry and capillary zone electrophoresis. The feasible sample pretreatment consists of a single extraction with a pH 2.5 phosphate buffer, centrifugation and dilution. For the spectrophotometric assay, the fourth derivative of the absorbance was used which gave satisfactory results in terms of accuracy (mean recovery 99.7%) and precision (mean RSD 3.4%). The electrophoretic experiments were carried out using the shortest effective length of the capillary (8.5 cm) in order to obtain a very rapid separation of reboxetine and dibenzepine used as the internal standard. Using a pH 2.5, 50 mM phosphate buffer as the background electrolyte, each analysis lasted less than 2.5 min. Accuracy (101.3%) and precision (1.5%) were very good. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:209 / 215
页数:7
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