Controlled release of bone morphogenetic protein 2 and dexamethasone loaded in core-shell PLLACL-collagen fibers for use in bone tissue engineering

被引：208

作者：

Su, Yan ^{[1
,2
,3
]}

Su, Qianqian ^{[4
]}

Liu, Wei ^{[1
,2
,3
]}

Lim, Marcus ^{[3
]}

Venugopal, Jayarama Reddy ^{[3
]}

Mo, Xiumei ^{[1
,2
,5
]}

Ramakrishna, Seeram ^{[1
,3
,5
]}

Al-Deyab, Salem S. ^{[5
]}

El-Newehy, Mohamed ^{[5
,6
]}

机构：

[1] Donghua Univ, Biomat & Tissue Engn Lab, Coll Chem & Chem Engn & Biotechnol, Shanghai 201620, Peoples R China

[2] Donghua Univ, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China

[3] Natl Univ Singapore, Fac Engn, Nanosci & Nanotechnol Initiat, HEM Lab, Singapore 117576, Singapore

[4] Natl Univ Singapore, Dept Chem, Singapore 117576, Singapore

[5] King Saud Univ, Coll Sci, Dept Chem, Petrochem Res Chair, Riyadh 11451, Saudi Arabia

[6] Tanta Univ, Fac Sci, Dept Chem, Tanta 31527, Egypt

来源：

ACTA BIOMATERIALIA | 2012年 / 8卷 / 02期

基金：

美国国家科学基金会;

关键词：

Coaxial electrospinning; Nanofibers; Bone morphogenetic protein 2; Dexamethasone; Controlled release; OSTEOGENIC DIFFERENTIATION; CROSS-LINKING; ELECTROSPUN; SCAFFOLDS; CELLS; GRAFT; FABRICATION; NANOFIBERS; DELIVERY; HEPARIN;

D O I：

10.1016/j.actbio.2011.11.002

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Electrospun nanofibers mimic the native extracellular matrix of bone and have generated considerable interest in bone tissue regeneration. The aim of this study was to fabricate novel poly(L-lactide-co-caprolactone) (PLLACL), PLLACL/collagen nanofibers blended with bone morphogenetic protein 2 (BMP2) and dexamethasone (DEX) for controlled release during bone tissue engineering (BTE). The morphology, surface hydrophilicity, and mechanical properties of the PLLACL/collagen nanofibrous mats were analyzed by scanning electron microscopy and water contact angle and mechanical stability determination. The performance of the scaffolds was investigated in terms of the viability and morphology of human mesenchymal stromal cells (hMSC) on the nanofibrous mats. BMP2 and DEX were successfully incorporated into PLLACL/collagen nanofibers by means of blending or coaxial electrospinning and the PLLACL/collagen blended fibers proved useful for hMSC culture. Release of the two growth factors from PLLACL/collagen nanofibrous mats in vitro was investigated by UV spectrophotometry. The release profiles for core-shell nanofibers showed more controlled release of the growth factors compared with the blended electrospun fibers. The experimental results show that controlled release of BMP2 and DEX can induce hMSC to differentiate into osteogenic cells for bone tissue engineering. The results imply that PLLACL/collagen nanofibers encapsulating two drugs and/or proteins have great potential in bone tissue engineering. Crown Copyright (C) 2011 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. All rights reserved.

引用

页码：763 / 771

页数：9