A new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant

被引：5

作者：

Mascini, EM ^{[1
]}

Hazenberg, MAJ ^{[1
]}

Verhage, LAE ^{[1
]}

Holm, SE ^{[1
]}

Verhoef, J ^{[1
]}

vanDijk, H ^{[1
]}

机构：

[1] UMEA UNIV,DEPT CLIN BACTERIOL,UMEA,SWEDEN

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1996年 / 3卷 / 06期

关键词：

D O I：

10.1128/CDLI.3.6.779-781.1996

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (greater than or equal to 99%), and time.

引用

页码：779 / 781

页数：3

共 23 条

[1]

BETLEY MJ, 1992, CHEM IMMUNOL, V55, P1

[2] STAPHYLOCOCCAL AND STREPTOCOCCAL PYROGENIC TOXINS INVOLVED IN TOXIC SHOCK SYNDROME AND RELATED ILLNESSES [J].

BOHACH, GA ;

FAST, DJ ;

NELSON, RD ;

SCHLIEVERT, PM .

CRITICAL REVIEWS IN MICROBIOLOGY, 1990, 17 (04) :251-272

[3] FURTHER PURIFICATION OF GROUP-A STREPTOCOCCAL PYROGENIC EXOTOXIN AND CHARACTERIZATION OF PURIFIED TOXIN [J].

CUNNINGHAM, CM ;

BARSUMIAN, EL ;

WATSON, DW .

INFECTION AND IMMUNITY, 1976, 14 (03) :767-775

[4]

FERETTI JJ, 1990, P AM SOC MICR C STRE

[5] PURIFICATION AND CHARACTERIZATION OF ERYTHROGENIC TOXINS .1. INVESTIGATION OF ERYTHROGENIC TOXIN-A PRODUCED BY STREPTOCOCCUS-PYOGENES STRAIN NY-5 [J].

GERLACH, D ;

KNOLL, H ;

KOHLER, W .

ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, 1980, 247 (02) :177-191

[6] MOLECULAR ANALYSIS OF PYROGENIC EXOTOXINS FROM STREPTOCOCCUS-PYOGENES ISOLATES ASSOCIATED WITH TOXIC SHOCK-LIKE SYNDROME [J].

HAUSER, AR ;

STEVENS, DL ;

KAPLAN, EL ;

SCHLIEVERT, PM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (08) :1562-1567

[7] ASPECTS OF PATHOGENESIS OF SERIOUS GROUP-A STREPTOCOCCAL INFECTIONS IN SWEDEN, 1988-1989 [J].

HOLM, SE ;

NORRBY, A ;

BERGHOLM, AM ;

NORGREN, M .

JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (01) :31-37

[8] PURIFICATION AND CHARACTERIZATION OF STREPTOCOCCAL ERYTHROGENIC TOXIN TYPE-A PRODUCED BY STREPTOCOCCUS-PYOGENES STRAIN NY-5 CULTURED IN THE SYNTHETIC MEDIUM NCTC-135 - COMPARISON WITH THE DIALYZED MEDIUM (TP MEDIUM) DERIVED TOXIN [J].

KAMEZAWA, Y ;

NAKAHARA, T .

MICROBIOLOGY AND IMMUNOLOGY, 1989, 33 (03) :183-194

[9]

KIM Y B, 1970, Journal of Experimental Medicine, V131, P611, DOI 10.1084/jem.131.3.611

[10] PURIFICATION AND CHARACTERIZATION OF ERYTHROGENIC TOXINS .4. MITOGENIC ACTIVITY OF ERYTHROGENIC TOXIN PRODUCED BY STREPTOCOCCUS-PYOGENES STRAIN NY-5 [J].

KNOLL, H ;

HRIBALOVA, V ;

GERLACH, D ;

KOHLER, W .

ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, 1981, 251 (01) :15-26

← 1 2 3 →