Wheat Fermentation With Enterococcus mundtii QAUSD01 and Wickerhamomyces anomalus QAUWA03 Consortia Induces Concurrent Gliadin and Phytic Acid Degradation and Inhibits Gliadin Toxicity in Caco-2 Monolayers

被引：12

作者：

Sakandar, Hafiz Arbab ^{[1
,2
]}

Kubow, Stan ^{[1
]}

Azadi, Behnam ^{[1
]}

Faryal, Rani ^{[2
]}

Ali, Barkat ^{[3
]}

Ghazanfar, Shakira ^{[4
]}

Quraishi, Umar Masood ^{[5
]}

Imran, Muhammad ^{[2
]}

机构：

[1] McGill Univ, Sch Human Nutr, Fac Agr & Environm Sci, Ste Anne De Bellevue, PQ, Canada

[2] Quaid I Azam Univ, Fac Biol Sci, Dept Microbiol, Islamabad, Pakistan

[3] Natl Agr Res Ctr, Food Sci Res Inst, Islamabad, Pakistan

[4] Natl Agr Res Ctr, NIGAB, Islamabad, Pakistan

[5] Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad, Pakistan

来源：

FRONTIERS IN MICROBIOLOGY | 2019年 / 9卷

关键词：

yeast; bacteria; fermentation; Caco-2; celiac; BEAN PHASEOLUS-VULGARIS; SOURDOUGH; BACTERIA; PROTEIN; FRACTIONS; DIGESTIBILITY; PROTEOLYSIS; PREVALENCE; PHYTATE; FLOURS;

D O I：

10.3389/fmicb.2018.03312

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Foods containing high amounts of either phytic acid or gliadin can pose a risk for development of iron deficiency and celiac disease, respectively. The present study was conducted to evaluate the effects of preselected gliadin degrading strains, Enterococcus mundtii QAUSD01 and Wickerhamomyces anomalus QAUWA03, on phytic acid and gliadin degradation in six wheat cultivars (Lasani 2008, Seher 2006, Chakwal 97, Shafaq 2006, Bars 2009, Barani 83). Tight junction proteins, trans-epithelial resistance (TER) and ruffle formation in Caco-2 cells were evaluated relative to Saccharomyces cerevisiae-mediated fermented and unfermented controls. Phytic acid degradation was demonstrated in all six cultivars fermented with E. mundtii QAUSD01 and W anomalus QAUWA03 consortia. Among the six fermented cultivars, Shafaq 2006 showed relatively higher degradation of gliadin. In comparison to the other tested wheat varieties, fermentation of Lasani 2006 was associated with minimal toxic effects on Caco-2 cells in terms of ruffle formation, tight junction proteins and TER, which can be attributed to extensive degradation of toxic gliadin fragments.

引用

页数：12