Dynactin is essential for growth cone advance

被引：11

作者：

Abe, Takako K. ^{[1
]}

Honda, Takao ^{[1
]}

Takei, Kohtaro ^{[2
]}

Mikoshiba, Katsuhiko ^{[3
]}

Hoffman-Kim, Diane ^{[4
]}

Jay, Daniel G. ^{[5
]}

Kuwano, Ryozo ^{[1
]}

机构：

[1] Niigata Univ, Ctr Bioresource Based Res, Brain Res Inst, Chuo Ku, Niigata 9518585, Japan

[2] Yokohama City Univ, Grad Sch Med, Dept Mol Pharmacol & Neurobiol, Yokohama, Kanagawa 2360004, Japan

[3] RIKEN BSI, Wako, Saitama 3510198, Japan

[4] Brown Univ, Dept Mol Pharmacol Physiol & Biotechnol, Ctr Biomed Engn, Providence, RI 02912 USA

[5] Tufts Univ, Sch Med, Dept Physiol, Boston, MA 02111 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 03期

关键词：

axon guidance; CALI (chromophore-assisted laser inactivation); dynactin; growth cone motility; neurite extension;

D O I：

10.1016/j.bbrc.2008.05.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150(Glued), from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150(Glued), plays an essential role in growth cone advance. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：418 / 422

页数：5

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