Characterization of the lipopolysaccharide O-antigen of Cronobacter turicensis HPB3287 as a polysaccharide containing a 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-galacto-non-2-ulosonic acid (legionaminic acid) residue

被引：18

作者：

MacLean, Leann L. ^{[1
]}

Vinogradov, Evgeny ^{[1
]}

Pagotto, Franco ^{[2
]}

Perry, Malcolm B. ^{[1
]}

机构：

[1] CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

[2] Hlth Canada, Food Directorate, Hlth Prod & Food Branch, Bur Microbial Hazards, Ottawa, ON K1A 0K9, Canada

来源：

CARBOHYDRATE RESEARCH | 2011年 / 346卷 / 16期

关键词：

Cronobacter turicensis; Lipopolysaccharide; O-Antigen; Bacterial polysaccharide; ESCHERICHIA-COLI; ENTEROBACTER-SAKAZAKII; IDENTIFICATION; GENUS; FOOD;

D O I：

10.1016/j.carres.2011.09.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cronobacter turicensis, previously known as Enterobacter sakazakii, is a Gram-negative opportunistic foodborne pathogen that has been reported as a cause of life-threatening neonatal infections. From chemical and physical analyses involving composition analysis, methylation, two-dimensional high-resolution nuclear magnetic resonance, and mass spectrometry methods, the antigenic 0-polysaccharide in the smooth-type lipopolysaccharide of C. turicensis (strain HPB 3287) was determined to be a high molecular mass polymer of a repeating pentasaccharide unit composed of D-galactose, D-glucose, 2-acetamido-2-deoxy-D-galactose, and 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-galacto-non-2-ulosonic acid (leg-ionaminic acid), in a molar ratio 2:1:1:1, and having the structure: alpha-D-Glcp 1 down arrow 4 [-> 3)-beta-D-Galp-(1 -> 3)-beta-D-GalpNAc-(1 -> 4)-alpha-D-Galp-(1 -> 4)-alpha-Leg5Ac7Ac-(2 ->](0). (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2589 / 2594

页数：6

共 41 条

[1] Elucidation of the structure and characterization of the gene cluster of the O-antigen of Cronobacter sakazakii G2592, the reference strain of C. sakazakii O7 serotype [J].