Long intergenic noncoding RNA-p21 inhibits apoptosis by decreasing PUMA expression in non-small cell lung cancer

被引：20

作者：

Yang, Tao ^{[1
]}

Zhang, Wenjun ^{[1
]}

Wang, Li ^{[2
]}

Xiao, Chunyan ^{[1
]}

Guo, Bingling ^{[1
]}

Gong, Yi ^{[1
]}

Liang, Xiping ^{[1
]}

Huang, Dehong ^{[1
]}

Li, Qiying ^{[1
]}

Nan, Yingyu ^{[1
]}

Xiang, Ying ^{[1
]}

Shao, Jianghe ^{[1
]}

机构：

[1] Chongqing Univ, Chongqing Canc Inst, Chongqing Canc Hosp,Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis &, 181 Hanyu Rd, Chongqing 400030, Peoples R China

[2] Chongqing Gen Hosp, Dept Oncol, Chongqing, Peoples R China

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2019年 / 47卷 / 01期

关键词：

Long noncoding RNAs; lincRNA-p21; PUMA; non-small cell lung cancer; apoptosis; p53; PROLIFERATION; CARCINOMA; PROGRESSION; METASTASIS; ONCOGENE;

D O I：

10.1177/0300060518816592

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objective Long noncoding RNAs (lncRNAs) are important mediators in tumor progression. Long intergenic noncoding RNA-p21 (lincRNA-p21) participates in multiple biological processes. This study explored the role of lincRNA-p21 in human non-small cell lung cancer (NSCLC) progression and potential regulatory mechanisms. Methods LincRNA-p21 expression in NSCLC tissues and cell lines (A549, H1299, H1650, and NCI-H2087) was determined by quantitative real-time PCR. LincRNA-p21 overexpressing and sh-lincRNA-p21 lentiviral were respectively transfected into H1299 and A549 cells. Flow cytometry was used to measure apoptosis. Microarray analysis and RNA pull-down assay were used to predict the target genes of lincRNA-p21. Finally, PUMA siRNA and overexpressing PUMA were transfected into NSCLC cells, and the extent of cell apoptosis was measured. The protein expression levels of the relative genes were confirmed by western blot analysis. Results LincRNA-p21 was significantly upregulated in NSCLC tissues and cells. The upregulation of lincRNA-p21 considerably inhibited cell apoptosis while the downregulation of lincRNA-p21 showed the opposite effect. PUMA was a direct target gene of lincRNA-p21 and was negatively correlated with lincRNA-p21 in NSCLC specimens. The anti-apoptotic effect of lincRNA-p21 can be effectively attenuated by the upregulation of PUMA. Conclusion LincRNA-p21 is aberrantly upregulated in NSCLC and inhibits cell apoptosis by decreasing PUMA expression.

引用

页码：481 / 493

页数：13

共 50 条

[41] Analysis of Long Noncoding RNAs in Aila-Induced Non-Small Cell Lung Cancer Inhibition [J].

Chen, Lin ;

Wu, Cui ;

Wang, Heming ;

Chen, Sinuo ;

Ma, Danhui ;

Tao, Ye ;

Wang, Xingye ;

Luan, Yanhe ;

Wang, Tiedong ;

Shi, Yan ;

Song, Guangqi ;

Zhao, Yicheng ;

Dong, Xijun ;

Wang, Bingmei .

FRONTIERS IN ONCOLOGY, 2021, 11

[42] RETRACTED: Long noncoding RNA ZFAS1 acts as an oncogene by targeting miR-193a-3p in human non-small cell lung cancer (Retracted Article) [J].

Ge, H-B ;

Chen, S. ;

Huang, S-R ;

Zhu, J. .

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2019, 23 (15) :6516-6523

[43] Long noncoding RNA PXN-AS1-L promotes non-small cell lung cancer progression via regulating PXN [J].

Zhang, Zhifa ;

Peng, Zhaohui ;

Cao, Junying ;

Wang, Jiaqi ;

Hao, Yongyu ;

Song, Kai ;

Wang, Yan ;

Hu, Wei ;

Zhang, Xuesong .

CANCER CELL INTERNATIONAL, 2019, 19 (1)

[44] Up-regulation of long noncoding RNA uc.338 predicts poor survival in non-small cell lung cancer [J].

Tian, Yu ;

Feng, Yun .

CANCER BIOMARKERS, 2018, 22 (04) :781-785

[45] Long noncoding RNAs: new insights into non-small cell lung cancer biology, diagnosis and therapy [J].

Ricciuti, Biagio ;

Mencaroni, Clelia ;

Paglialunga, Luca ;

Paciullo, Francesco ;

Crino, Lucio ;

Chiari, Rita ;

Metro, Giulio .

MEDICAL ONCOLOGY, 2016, 33 (02)

[46] Low expression of LncRNAAFAP1-AS1 inhibits proliferation and promotes apoptosis of non-small cell lung cancer cells through inhibiting wnt signaling pathway [J].

Sun, Ying ;

Fan, Liming ;

Li, Yixian .

CELLULAR AND MOLECULAR BIOLOGY, 2023, 69 (12) :176-180

[47] Long Noncoding RNA ANRIL Regulates Proliferation of Non-small Cell Lung Cancer and Cervical Cancer Cells [J].

Naemura, Madoka ;

Murasaki, Chihiro ;

Inoue, Yasutoshi ;

Okamoto, Haruna ;

Kotake, Yojiro .

ANTICANCER RESEARCH, 2015, 35 (10) :5377-5382

[48] Long Noncoding RNA SNHG6 Promotes Proliferation and Inhibits Apoptosis in Non-small Cell Lung Cancer Cells by Regulating miR-490-3p/RSF1 Axis [J].

Dong, Zheng ;

Liu, Honglai ;

Zhao, Guangli .

CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2020, 35 (05) :351-361

[49] Bmi-1 expression modulates non-small cell lung cancer progression [J].

Xiong, Dan ;

Ye, Yunlin ;

Fu, Yujie ;

Wang, Jinglong ;

Kuang, Bohua ;

Wang, Hongbo ;

Wang, Xiumin ;

Zu, Lidong ;

Xiao, Gang ;

Hao, Mingang ;

Wang, Jianhua .

CANCER BIOLOGY & THERAPY, 2015, 16 (05) :756-763

[50] miR-512-5p induces apoptosis and inhibits glycolysis by targeting p21 in non-small cell lung cancer cells [J].

Chu, Kaili ;

Gao, Guanghui ;

Yang, Xiufang ;

Ren, Shengxiang ;

Li, Yao ;

Wu, Hai ;

Huang, Yan ;

Zhou, Caicun .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2016, 48 (02) :577-586

← 1 2 3 4 5 →