Isolation and characterization of polymorphic microsatellite loci from elongate loach (Leptobotia elongate), a threatened fish species endemic to the Yangtze River

被引：4

作者：

Liu, Hong Yan ^{[2
]}

Xiong, Fei ^{[2
]}

Duan, Xin Bin ^{[1
]}

Chen, Da Qing ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

[2] Jianghan Univ, Coll Life Sci, Wuhan 430056, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 01期

基金：

美国国家科学基金会;

关键词：

Elongate loach; Microsatellite; Polymorphism;

D O I：

10.1007/s12686-011-9491-7

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Elongate loach (Leptobotia elongate) is a threatened fish species endemic to the Yangtze River in China. A first set of 22 polymorphism microsatellite loci isolated from this species was characterized. The number of alleles, observed and expected heterozygosity ranged from 2 to 12, from 0.2667 to 1.000 and from 0.2391 to 0.9011, respectively. Seven loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. The polymorphism information content ranged from 0.204 to 0.857. Ten loci were highly informative, 11 were moderately informative, and one was slightly informative. These polymorphic microsatellite markers will be useful for genetic monitoring of natural populations and management of breeding stocks in hatcheries.

引用

页码：129 / 131

页数：3

共 11 条

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