Portable sequencing as a teaching tool in conservation and biodiversity research

被引:31
作者
Watsa, Mrinalini [1 ,2 ,3 ]
Erkenswick, Gideon A. [1 ,3 ,4 ]
Pomerantz, Aaron [5 ,6 ]
Prost, Stefan [7 ,8 ]
机构
[1] Field Projects Int, St Louis, MO 63119 USA
[2] San Diego Zoo Global, Populat Sustainabil, San Diego, CA 92101 USA
[3] Univ Missouri, Dept Biol, 8001 Nat Bridge Rd, St Louis, MO 63121 USA
[4] Washington Univ, Sch Med, Infect Dis, St Louis, MO 63110 USA
[5] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA
[6] Marine Biol Lab, Woods Hole, MA 02543 USA
[7] Senckenberg, LOEWE Ctr Translat Biodivers Genom, Frankfurt, Germany
[8] Natl Zool Garden, South African Natl Biodivers Inst, Pretoria, South Africa
关键词
D O I
10.1371/journal.pbio.3000667
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As biodiversity loss continues to accelerate, there is a critical need for education and biomonitoring across the globe. Portable technologies allow for in situ molecular biodiversity monitoring that has been historically out of reach for many researchers in habitat nations. In the realm of education, portable tools such as DNA sequencers facilitate in situ hands-on training in real-time sequencing and interpretation techniques. Here, we provide step-by-step protocols as a blueprint for a terrestrial conservation genetics field training program that uses low-cost, portable devices to conduct genomics-based training directly in biodiverse habitat countries.
引用
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页数:9
相关论文
共 20 条
[1]   Building local capacity for genomics research in Africa: recommendations from analysis of publications in Sub-Saharan Africa from 2004 to 2013 [J].
Adedokun, Babatunde O. ;
Olopade, Christopher O. ;
Olopade, Olufunmilayo I. .
GLOBAL HEALTH ACTION, 2016, 9
[2]  
[Anonymous], 2019, BIORXIV, DOI [10.1101/581728, DOI 10.1101/581728]
[3]   Approaching a state shift in Earth's biosphere [J].
Barnosky, Anthony D. ;
Hadly, Elizabeth A. ;
Bascompte, Jordi ;
Berlow, Eric L. ;
Brown, James H. ;
Fortelius, Mikael ;
Getz, Wayne M. ;
Harte, John ;
Hastings, Alan ;
Marquet, Pablo A. ;
Martinez, Neo D. ;
Mooers, Arne ;
Roopnarine, Peter ;
Vermeij, Geerat ;
Williams, John W. ;
Gillespie, Rosemary ;
Kitzes, Justin ;
Marshall, Charles ;
Matzke, Nicholas ;
Mindell, David P. ;
Revilla, Eloy ;
Smith, Adam B. .
NATURE, 2012, 486 (7401) :52-58
[4]  
Bhamla MS, PAPERFUGE ULTRALOW C, DOI [10.1101/072207, DOI 10.1101/072207]
[5]  
Blanco M.B., 2019, NEXT GENERATION IN S, DOI [10.1101/650614, DOI 10.1101/650614]
[6]   Defaunation in the Anthropocene [J].
Dirzo, Rodolfo ;
Young, Hillary S. ;
Galetti, Mauro ;
Ceballos, Gerardo ;
Isaac, Nick J. B. ;
Collen, Ben .
SCIENCE, 2014, 345 (6195) :401-406
[7]  
Ducluzeau A-L, 2019, INTRO DNA SEQUENCING
[8]   Teaching Biology in the Field: Importance, Challenges, and Solutions [J].
Fleischner, Thomas L. ;
Espinoza, Robert E. ;
Gerrish, Gretchen A. ;
Greene, Harry W. ;
Kimmerer, Robin Wall ;
Lacey, Eileen A. ;
Pace, Steven ;
Parrish, Julia K. ;
Swain, Hilary M. ;
Trombulak, Stephen C. ;
Weisberg, Saul ;
Winkler, David W. ;
Zander, Lisa .
BIOSCIENCE, 2017, 67 (06) :558-567
[9]   Genomics for All: International Open Science Genomics Projects and Capacity Building in the Developing World [J].
Hetu, Martin ;
Koutouki, Konstantia ;
Joly, Yann .
FRONTIERS IN GENETICS, 2019, 10
[10]  
Juul S, 2015, WHATS MY POT REAL TI, P030742