Type-3 copper proteins as biocompatible and reusable oxygen sensors

被引：15

作者：

Zauner, Gerhild ^{[1
]}

Strianese, Maria ^{[1
]}

Bubacco, Luigi ^{[2
]}

Aartsma, Thijs J. ^{[3
]}

Tepper, Armand W. J. W. ^{[1
]}

Canters, Gerard W. ^{[1
]}

机构：

[1] Leiden Univ, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands

[2] Univ Padua, Dept Biol, I-35121 Padua, Italy

[3] Leiden Univ, Leiden Inst Phys, NL-2300 RA Leiden, Netherlands

来源：

INORGANICA CHIMICA ACTA | 2008年 / 361卷 / 04期

关键词：

sensitised fluorescence; FRET; hemocyanin; sol-gel; oxygen sensor;

D O I：

10.1016/j.ica.2007.08.018

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Fluorescently labeled type-3 copper proteins have been proposed previously as solution oxygen sensors by using a FRET mechanism. Herein, we describe how this principle can be adapted to sense O-2 by means of proteins immobilized in optically transparent silica matrices. Specifically, the protein, hemocyanin from Octopus vulgaris N-terminally labeled with Cy5, is immobilized in two different kinds of optically transparent silica matrices, which appear to be a promising platform for enzyme encapsulation. The presented results provide proof of principle that fluorescently labeled proteins immobilized in a silica matrix can be implemented in a reusable, biocompatible and stable oxygen measuring device that might lead to new developments in the field of optical biosensing. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：1116 / 1121

页数：6

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