Fluorescence detection of cervical intraepithelial neoplasia for photodynamic therapy with the topical agents 5-aminolevulinic acid and benzoporphyrin-derivative monoacid ring

被引:23
作者
Keefe, KK
Chahine, EB
DiSaia, PJ
Krasieva, TB
Lin, F
Berns, MW
Tadir, Y
机构
[1] Univ Calif Irvine, Irvine Med Ctr, Dept Obstet & Gynecol, Orange, CA 92668 USA
[2] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Irvine, CA 92715 USA
[3] Dartmouth Coll, Hitchcock Med Ctr, Sch Med, Norris Cotton Canc Ctr, Lebanon, NH 03756 USA
[4] Columbia Presbyterian Med Ctr, Nyack Hosp, Nyack, NY USA
关键词
5-aminolevulinic acid; benzoporphyrin-derivative monoacid ring; cervical dysplasia; cervical intraepithelial neoplasia; cervix; photodynamic therapy; protoporphyrin IX;
D O I
10.1067/mob.2001.113123
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: The aim of this study was to determine whether 2 photosensitizers, benzoporphyrin-derivative monoacid ring and 5-aminolevulinic acid, are selectively absorbed by dysplastic cervical cells after topical administration. STUDY DESIGN: This phase I clinical trial involved 18 women with biopsy-proven cervical intraepithelial neoplasia at the Beckman Laser Institute, [Nine, Calif. Colposcopically directed cervical biopsy specimens obtained after 1.5, 3, or 6 hours of exposure to a randomly assigned photosensitizer were evaluated for selective drug absorption with hematoxylin and eosin staining and fluorescence microscopy. RESULTS: After exposure to 5-aminolevulinic acid, cervical tissue showed maximal fluorescence in dysplastic cells relative to normal cells, with negligible stroma[ fluorescence. According to our detection methods benzoporphyrin-derivative monoacid ring demonstrated nonselective, diffusion-driven uptake, with fluorescence appearing in the superficial cells, followed by nonselective drug absorption in the remaining cells and stroma of the epithelium. CONCLUSION: Our data demonstrated selective absorption of 5-aminolevulinic acid by dysplastic cervical cells. This agent therefore represents a promising photosensitizing prodrug for the treatment of cervical intraepithelia[ neoplasia with photodynamic therapy.
引用
收藏
页码:1164 / 1169
页数:6
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