DNA photoligation in two-color detection of DNA point mutation

被引:5
作者
Ami, Takehiro
Ozaki, Genki
Yoshimura, Yoshinaga
Fujimoto, Kenzo [1 ]
机构
[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi 9231292, Japan
关键词
D O I
10.1246/cl.2008.134
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report a method for the rapid differentiation of nucleic acid sequences with 5-carboxyvinyl-2'-deoxyuridine that allows the simultaneous observation of two colors and facilitates the SNP typing of a heterozygous sample.
引用
收藏
页码:134 / 135
页数:2
相关论文
共 20 条
  • [1] A haplotype map of the human genome
    Altshuler, D
    Brooks, LD
    Chakravarti, A
    Collins, FS
    Daly, MJ
    Donnelly, P
    Gibbs, RA
    Belmont, JW
    Boudreau, A
    Leal, SM
    Hardenbol, P
    Pasternak, S
    Wheeler, DA
    Willis, TD
    Yu, FL
    Yang, HM
    Zeng, CQ
    Gao, Y
    Hu, HR
    Hu, WT
    Li, CH
    Lin, W
    Liu, SQ
    Pan, H
    Tang, XL
    Wang, J
    Wang, W
    Yu, J
    Zhang, B
    Zhang, QR
    Zhao, HB
    Zhao, H
    Zhou, J
    Gabriel, SB
    Barry, R
    Blumenstiel, B
    Camargo, A
    Defelice, M
    Faggart, M
    Goyette, M
    Gupta, S
    Moore, J
    Nguyen, H
    Onofrio, RC
    Parkin, M
    Roy, J
    Stahl, E
    Winchester, E
    Ziaugra, L
    Shen, Y
    [J]. NATURE, 2005, 437 (7063) : 1299 - 1320
  • [2] SNPing in the human genome
    Carlson, CS
    Newman, TL
    Nickerson, DA
    [J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (01) : 78 - 85
  • [3] Faster, better, cheaper genotyping
    Chicurel, M
    [J]. NATURE, 2001, 412 (6847) : 580 - 582
  • [4] A DNA polymorphism discovery resource for research on human genetic variation
    Collins, FS
    Brooks, LD
    Chakravarti, A
    [J]. GENOME RESEARCH, 1998, 8 (12) : 1229 - 1231
  • [5] Pyrosequencing™:: An accurate detection platform for single nucleotide polymorphisms
    Fakhrai-Rad, H
    Pourmand, N
    Ronaghi, M
    [J]. HUMAN MUTATION, 2002, 19 (05) : 479 - 485
  • [6] Template-directed photoreversible ligation of deoxyoligonucleotides via 5-vinyldeoxyuridine
    Fujimoto, K
    Matsuda, S
    Takahashi, N
    Saito, I
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (23) : 5646 - 5647
  • [7] Resequencing and mutational analysis using oligonucleotide microarrays
    Hacia, JG
    [J]. NATURE GENETICS, 1999, 21 (Suppl 1) : 42 - 47
  • [8] Howell WM, 1999, NAT BIOTECHNOL, V17, P87, DOI 10.1038/5270
  • [9] Methods for genotyping single nucleotide polymorphisms
    Kwok, PY
    [J]. ANNUAL REVIEW OF GENOMICS AND HUMAN GENETICS, 2001, 2 : 235 - 258
  • [10] Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes
    Lyamichev, V
    Mast, AL
    Hall, JG
    Prudent, JR
    Kaiser, MW
    Takova, T
    Kwiatkowski, RW
    Sander, TJ
    de Arruda, M
    Arco, DA
    Neri, BP
    Brow, MAD
    [J]. NATURE BIOTECHNOLOGY, 1999, 17 (03) : 292 - 296