Synthesis of Aloe vera-conjugated silver nanoparticles for use against multidrug-resistant microorganisms

Background: The emergence of multidrug-resistant (MDR) microorganisms is one of the biggest and most challenging public health issues drawing considerable attention of the scientific community. Here, we present an easy, one-step, inexpensive and ecofriendly/biologically mediated synthesis of Aloe vera- conjugated silver nanoparticles (Av-AgNPs) where the aqueous plant extract acts as a reducing and stabilizing agent and the resultant conjugate exhibits remarkable potential to limit/inhibit the growth of MDR pathogens. Results: The nanosynthesis concluded in 4-6 h at 65 degrees C and was followed by detailed characterization of the bioconjugated Av-AgNPs (with and without fabrication on cellulosic materials i.e., cotton fabric and filter paper) using a combination of UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), x-ray diffraction (XRD) and inductively coupled plasma optical emission spectroscopy (ICP-OES). The so-characterized NPs showed growth inhibitory effects on multiple strains including the Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli (E. coli), Acinetobacter baumannii (A. baumannii), Pseudomonas aeruginosa (P. pseudomonas) and, more importantly, the fungus Candida albicans (C. albicans), when analyzed using the Kirby-Bauer method. A notable reduction in the colony-forming unit (CFU) counts of the E. coli (present in contaminated drinking water) was also observed when the filter paper encrusted with Av-AgNPs was applied as a filtration material. Conclusions: In conclusion, the biofabricated Av-AgNPs are easy to synthesize and are a cost-effective alternative to inorganic AgNPs, with considerable antimicrobial activity, deserving further investigations for biomedical applications. How to cite: Arshad H, Saleem M, Pasha U, et al. Synthesis of Aloe vera-conjugated silver nanoparticles for use against multidrug-resistant microorganisms. Electron J Biotechnol 2022;55. https://doi.org/10.1016/j. ejbt.2021.11.003 (c) 2021 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).