Purification and molecular cloning of an intracellular 3-hydroxybutyrate-oligomer hydrolase from Paucimonas lemoignei

被引：2
作者：
Uchino, Keilchi
Katsumata, Yoko
Takeda, Tornoko
Arai, Hiroki
Shiraki, Mari
Saito, Terumi
机构：
[1] Kanagawa Univ, Fac Sci, Dept Biol Sci, Mol Microbiol Lab, Hiratsuka, Kanagawa 2591293, Japan
[2] Kanagawa Univ, Res Inst Integral Sci, Hiratsuka, Kanagawa 2591293, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2007年 / 104卷 / 03期
关键词：
3-hydroxybutyrate-oligomer hydrolase; Paucimonas lemoignei; purification; gene cloning; PhaZc;
D O I：
10.1263/jbb.104.224
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An intracellular 3-hydroxybutyrate-oligomer hydrolase was purified from a poly(3-hydroxybutyrate)-degrading bacterium, Paucimonas lemoignei. It hydrolyzed the 3-hydroxybutyrate dimer with the highest specific activity of any of the enzymes reported so far. The gene was cloned and sequenced. The deduced amino acid sequence showed that the enzyme is a homolog of the PhaZc of Ralstonia eutropha H16.
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页码：224 / 226
页数：3
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