Cellular anatomy of the mouse primary motor cortex

被引：119

作者：

Munoz-Castaneda, Rodrigo ^{[1
]}

Zingg, Brian ^{[2
,3
]}

Matho, Katherine S. ^{[1
]}

Chen, Xiaoyin ^{[1
,4
]}

Wang, Quanxin ^{[4
]}

Foster, Nicholas N. ^{[2
,3
]}

Li, Anan ^{[5
,6
]}

Narasimhan, Arun ^{[1
]}

Hirokawa, Karla E. ^{[4
,7
]}

Huo, Bingxing ^{[1
]}

Bannerjee, Samik ^{[1
]}

Korobkova, Laura ^{[3
]}

Park, Chris Sin ^{[8
]}

Park, Young-Gyun ^{[9
]}

Bienkowski, Michael S. ^{[3
,10
]}

Chon, Uree ^{[11
]}

Wheeler, Diek W. ^{[12
,13
]}

Li, Xiangning ^{[5
,6
]}

Wang, Yun ^{[4
]}

Naeemi, Maitham ^{[4
]}

Xie, Peng ^{[14
]}

Liu, Lijuan ^{[14
]}

Kelly, Kathleen ^{[1
]}

An, Xu ^{[1
,15
]}

Attili, Sarojini M. ^{[12
,13
]}

Bowman, Ian ^{[2
,3
]}

Bludova, Anastasiia ^{[1
]}

Cetin, Ali ^{[4
]}

Ding, Liya ^{[14
]}

Drewes, Rhonda ^{[1
]}

D'Orazi, Florence ^{[4
]}

Elowsky, Corey ^{[1
]}

Fischer, Stephan ^{[1
]}

Galbavy, William ^{[1
]}

Gao, Lei ^{[2
,3
]}

Gillis, Jesse ^{[1
]}

Groblewski, Peter A. ^{[4
]}

Gou, Lin ^{[2
,3
]}

Hahn, Joel D. ^{[16
]}

Hatfield, Joshua T. ^{[1
,15
]}

Hintiryan, Houri ^{[2
,3
]}

Huang, Junxiang Jason ^{[17
]}

Kondo, Hideki ^{[1
]}

Kuang, Xiuli ^{[18
]}

Lesnar, Philip ^{[4
]}

Li, Xu ^{[1
]}

Li, Yaoyao ^{[18
]}

Lin, Mengkuan ^{[1
]}

Lo, Darrick ^{[2
,3
]}

Mizrachi, Judith ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Neurobiol, Los Angeles, CA 90095 USA

[3] Univ Southern Calif, Keck Sch Med, USC Stevens Neuroimaging & Informat Inst INI, USC, Los Angeles, CA 90007 USA

[4] Allen Inst Brain Sci, Seattle, WA 98109 USA

[5] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, MoE Key Lab Biomed Photon, Wuhan, Peoples R China

[6] JITRI, HUST Suzhou Inst Brainsmat, Suzhou, Peoples R China

[7] Cajal Neurosci, Seattle, WA 98102 USA

[8] Univ Calif Los Angeles, David Geffen Sch Med, Ctr Neurobehav Genet,Dept Psychiat & Biobehav Sci, Jane & Terry Semel Inst Neurosci & Human Behav, Los Angeles, CA 90095 USA

[9] MIT, Dept Chem Engn, Inst Med Engn & Sci, Picower Inst Learning & Memory, Cambridge, MA 02139 USA

[10] Univ Southern Calif, Keck Sch Med, USC, Dept Physiol & Neurosci,Zilkha Neurogenet Inst, Los Angeles, CA 90007 USA

[11] Penn State Univ, Coll Med, Dept Neural & Behav Sci, Hershey, PA USA

[12] George Mason Univ, Ctr Neural Informat Struct & Plast, Bioengn Dept, Fairfax, VA 22030 USA

[13] George Mason Univ, Krasnow Inst Adv Study, Fairfax, VA 22030 USA

[14] Southeast Univ, SEU ALLEN Joint Ctr, Inst Brain & Intelligence, Nanjing, Peoples R China

[15] Duke Univ, Sch Med, Dept Neurobiol, Durham, NC 27708 USA

[16] Univ Southern Calif, Dept Biol Sci, Los Angeles, CA 90007 USA

[17] Univ Southern Calif, Ctr Neural Circuits & Sensory Proc Disorders, Zilkha Neurogenet Inst ZNI, Dept Physiol & Neurosci,Keck Sch Med, Los Angeles, CA 90007 USA

[18] Wenzhou Med Univ, Sch Optometry & Ophthalmol, Wenzhou, Peoples R China

[19] Shanghai Univ, Sch Comp Engn & Sci, Shanghai, Peoples R China

[20] Univ Calif San Diego, Neurobiol Sect, Div Biol Sci, San Diego, CA 92103 USA

[21] Univ Penn, Dept Radiol, Philadelphia, PA 19104 USA

来源：

NATURE | 2021年 / 598卷 / 7879期

关键词：

GABAERGIC NEURONS; DRIVER LINES; ORGANIZATION; RESOLUTION; INPUT; MICROSTIMULATION; CONNECTIVITY; TOMOGRAPHY; MORPHOLOGY; CONNECTOME;

D O I：

10.1038/s41586-021-03970-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated and interpreted(1). Here, as part of the collaborative Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based anatomical description of one exemplar brain structure, the mouse primary motor cortex, upper limb area (MOp-ul). Usinggenetic and viral labelling, barcoded anatomy resolved by sequencing, single-neuron reconstruction, whole-brain imaging and cloud-based neuroinformaticstools, we delineated the MOp-ul in 3D and refined its sublaminar organization. We defined around two dozen projection neuron types in the MOp-ul and derived an inputoutput wiring diagram, which will facilitate future analyses of motor control circuitry across molecular, cellular and system levels. This work provides a roadmap towards a comprehensive cellular-resolution description of mammalian brain architecture.

引用

页码：159 / +

页数：45

共 85 条

[1] Abramoff M. D., 2004, BIOPHOTONICS INT, V11, P36, DOI DOI 10.1201/9781420005615.AX4
[2] Ahrens MB, 2013, NAT METHODS, V10, P413, DOI [10.1038/nmeth.2434, 10.1038/NMETH.2434]
[3] A whole-brain atlas of monosynaptic input targeting four different cell types in the medial prefrontal cortex of the mouse
Ahrlund-Richter, Sofie
Xuan, Yang
van Lunteren, Josina Anna
Kim, Hoseok
Ortiz, Cantin
Dorocic, Iskra Pollak
Meletis, Konstantinos
Carlen, Marie
[J]. NATURE NEUROSCIENCE, 2019, 22 (04) : 657 - +
[4] An open repository for single-cell reconstructions of the brain forest
Akram, Masood A.
Nanda, Sumit
Maraver, Patricia
Armananzas, Ruben
Ascoli, Giorgio A.
[J]. SCIENTIFIC DATA, 2018, 5
[5] Operations research methods for estimating the population size of neuron types
Attili, Sarojini M.
Mackesey, Sean T.
Ascoli, Giorgio A.
[J]. ANNALS OF OPERATIONS RESEARCH, 2020, 289 (01) : 33 - 50
[6] Fast unfolding of communities in large networks
Blondel, Vincent D.
Guillaume, Jean-Loup
Lambiotte, Renaud
Lefebvre, Etienne
[J]. JOURNAL OF STATISTICAL MECHANICS-THEORY AND EXPERIMENT, 2008,
[7] BRAIN Initiative Cell Census Network (BICCN), 2021, NATURE, V598, P86, DOI [10.1038/s41586-021-03950-0(2021), DOI 10.1038/S41586-021-03950-0(2021)]
[8] TeraFly: real-time three-dimensional visualization and annotation of terabytes of multidimensional volumetric images
Bria, Alessandro
Iannello, Giulio
Onofri, Leonardo
Peng, Hanchuan
[J]. NATURE METHODS, 2016, 13 (03) : 192 - 194
[9] Quantifying neuronal size: Summing up trees and splitting the branch difference
Brown, Kerry M.
Gillette, Todd A.
Ascoli, Giorgio A.
[J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2008, 19 (06) : 485 - 493
[10] High-Throughput Mapping of Long-Range Neuronal Projection Using In Situ Sequencing
Chen, Xiaoyin
Sun, Yu-Chi
Zhan, Huiqing
Kebschull, Justus M.
Fischer, Stephan
Matho, Katherine
Huang, Z. Josh
Gillis, Jesse
Zador, Anthony M.
[J]. CELL, 2019, 179 (03) : 772 - +

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