Solid-phase microextraction for assessment of plasma protein binding, a complement to rapid equilibrium dialysis

被引：6

作者：

Ahmad, Sheelan ^{[1
,2
]}

Baker, Daniel ^{[2
]}

Murnane, Darragh ^{[2
]}

Spooner, Neil ^{[2
,3
]}

Gerhard, Ute ^{[2
]}

机构：

[1] GlaxoSmithKline, Res & Dev, Stevenage, Herts, England

[2] Univ Hertfordshire, Sch Life & Med Sci Pharmaceut & Biol Sci, Dept Clin, Hatfield, Herts, England

[3] Spooner Bioanalyt Solut Ltd, Hertford, England

来源：

BIOANALYSIS | 2021年 / 13卷 / 14期

关键词：

free concentration; microsampling; plasma protein binding; rapid equilibrium dialysis; solid-phase microextraction; DRUGS; ULTRACENTRIFUGATION; ULTRAFILTRATION;

D O I：

10.4155/bio-2021-0109

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: Determination of plasma protein binding (PPB) is considered vital for better understanding of pharmacokinetic and pharmacodynamic activities of drugs due to the role of free concentration in pharmacological response. Methodology & results: Solid-phase microextraction (SPME) was investigated for measurement of PPB from biological matrices and compared with a gold standard approach (rapid equilibrium dialysis [RED]). Discussion & conclusion: SPME-derived values of PPB correlated well with literature values, and those determined by RED. Respectively, average protein binding across three concentrations by RED and SPME was 33.1 and 31.7% for metoprolol, 89.0 and 86.6% for propranolol and 99.2 and 99.0% for diclofenac. This study generates some evidence for SPME as an alternative platform for the determination of PPB.

引用

页码：1101 / 1111

页数：11

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