Isolation and characterization of microsatellite loci from the entomopathogenic fungus Beauveria bassiana (Ascomycota: Hypocreales)

被引:51
作者
Rehner, SA [1 ]
Buckley, EP [1 ]
机构
[1] USDA ARS, Insect Biocontrol Lab, Beltsville, MD 20705 USA
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 03期
关键词
ascomycete; Beauveria bassiana; fungi; Hypocreales; microsatellite; population genetics;
D O I
10.1046/j.1471-8286.2003.00464.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Beauveria bassiana, an entomogenous fungus used for the biological control of pest insects, comprises a globally-distributed species complex of regionally endemic lineages. In order to study the population genetics of B. bassiana, detail species boundaries, conduct ecological studies of natural populations and track fates of experimentally-released strains, sensitive genetic markers are required. We describe the isolation and characterization of eight microsatellite loci that amplify successfully from strains representative of the phylogenetic diversity in the B. bassiana complex.
引用
收藏
页码:409 / 411
页数:3
相关论文
共 10 条
[1]  
CAMBERERI EB, 1994, FUNGAL GENETICS NEWS, V40, P22
[2]   Allelic variation of a Beauveria bassiana (Ascomycota: Hypocreales) minisatellite is independent of host range and geographic origin [J].
Coates, BS ;
Hellmich, RL ;
Lewis, LC .
GENOME, 2002, 45 (01) :125-132
[3]  
Edwards KJ, 1996, BIOTECHNIQUES, V20, P758
[4]   Strain-specific microsatellite markers in the entomopathogenic fungus Beauveria brongniartii [J].
Enkerli, J ;
Widmer, F ;
Gessler, C ;
Keller, S .
MYCOLOGICAL RESEARCH, 2001, 105 :1079-1087
[5]  
GLENN TC, 2000, MICROSATELLITE 2000
[6]  
Goettel Mark S., 1997, P213, DOI 10.1016/B978-012432555-5/50013-0
[7]   Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites [J].
Hamilton, MB ;
Pincus, EL ;
Di Fiore, A ;
Fleischer, RC .
BIOTECHNIQUES, 1999, 27 (03) :500-+
[8]   Discovery-and demonstration of the teleomorph of Beauveria bassiana (Bals.) Vuill., an important entomogenous fungus [J].
Li, ZZ ;
Li, CR ;
Huang, B ;
Fan, MZ .
CHINESE SCIENCE BULLETIN, 2001, 46 (09) :751-753
[9]   AN OPTIMIZED FREEZE-SQUEEZE METHOD FOR THE RECOVERY OF DNA FRAGMENTS FROM AGAROSE GELS [J].
TAUTZ, D ;
RENZ, M .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :14-19
[10]   PREPARATIVE AND ANALYTICAL PURIFICATION OF DNA FROM AGAROSE [J].
VOGELSTEIN, B ;
GILLESPIE, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (02) :615-619