Reference gene selection for quantitative real-time polymerase chain reaction in Populus

被引：126

作者：

Xu, Meng ^{[1
,2
]}

Zhang, Bo ^{[1
,2
]}

Su, Xiaohua ^{[3
]}

Zhang, Shougong ^{[3
]}

Huang, Minren ^{[1
,2
]}

机构：

[1] Nanjing Forestry Univ, Jiangsu Key Lab Poplar Germplasm Enhancement & Va, Nanjing 210037, Peoples R China

[2] Nanjing Forestry Univ, Key Lab Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China

[3] Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 408卷 / 02期

关键词：

RT-PCR; EXPRESSION; GENOME; RNA;

D O I：

10.1016/j.ab.2010.08.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.

引用

页码：337 / 339

页数：3

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