Differential Expression of Cyclic Nucleotide Phosphodiesterases 4 in Developing Rat Lung

被引:5
|
作者
Lopez, Emmanuel [2 ,3 ,4 ]
Jarreau, Pierre-Henri [2 ,3 ,4 ]
Zana, Elodie [3 ]
Franco-Montoya, Marie-Laure [3 ,5 ]
Schmitz, Thomas [2 ,3 ,6 ]
Evain-Brion, Daniele [3 ]
Bourbon, Jacques [3 ,5 ]
Delacourt, Christophe [3 ,5 ]
Mehats, Celine [1 ,3 ]
机构
[1] Hop St Vincent de Paul, Inserm Paris Descartes, UMR 767, F-75014 Paris, France
[2] Univ Paris 05, Fac Med, Paris, France
[3] PremUP, Paris, France
[4] Grp Hosp Cochin St Vincent de Paul, AP HP, Serv Med Neonatale Port Royal, Paris, France
[5] Hop Henri Mondor, INSERM, U955, F-94010 Creteil, France
[6] Grp Hosp Cochin St Vincent de Paul, AP HP, Serv Obstet, Paris, France
关键词
lung development; phosphodiesterases; 4; cyclic AMP; DEPENDENT PROTEIN-KINASE; GENE-EXPRESSION; MOLECULAR REGULATION; PDE4D; PHOSPHORYLATION; INHIBITION; RECEPTORS; ONTOGENY; ANALOGS;
D O I
10.1002/dvdy.22374
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
During the perinatal period, lungs undergo changes to adapt to air breathing. The genes involved in these changes are developmentally regulated by various signaling pathways, including the cyclic nucleotide cAMP. As PDE4s are critical enzymes for regulation of cAMP levels, the objective of this study was to investigate PDE4's ontogeny in developing rat lung during the perinatal period. Pulmonary PDE4 activity, PDE4A-D, PDE4B, and PDE4D variant expression levels, PDE4B and PDE4D protein levels, and PDE4D localization in distal lung were determined. PDE4 activity increased towards term, dropped at birth, and increased thereafter to reach a plateau at the end of the second week of life. PDE4B2 and PDE4D long forms demonstrated a pattern of expression that increased markedly at birth. After birth, PDE4D was expressed in alveolar epithelial and mesenchymal cells. The study, therefore, evidenced striking variations in expression patterns among the PDE4 family that differed from changes in global PDE4 activity. Developmental Dynamics 239:2470-2478, 2010. (c) 2010 Wiley-Liss, Inc.
引用
收藏
页码:2470 / 2478
页数:9
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