Determining isoleucine side-chain rotamer-sampling in proteins from 13C chemical shift

被引：12

作者：

Siemons, Lucas ^{[1
]}

Uluca-Yazgi, Boran ^{[2
,3
]}

Pritchard, Ruth B. ^{[1
]}

McCarthy, Stephen ^{[4
]}

Heise, Henrike ^{[2
,3
]}

Hansen, D. Flemming ^{[1
]}

机构：

[1] UCL, Inst Struct & Mol Biol, Div Biosci, London WC1E 6BT, England

[2] Heinrich Heine Univ Dusseldorf, Inst Phys Biol, Dusseldorf, Germany

[3] Forschungszentrum Julich, Inst Complex Syst, ICS 6 Struct Biochem & JuStruct Julich Ctr Struct, Julich, Germany

[4] UCL, Dept Chem, 20 Gordon St, London WC1H 0AJ, England

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 94期

基金：

英国惠康基金; 英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

DYNAMICS; COUPLINGS; BACKBONE; STATES;

D O I：

10.1039/c9cc06496f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Chemical shifts are often the only nuclear magnetic resonance parameter that can be obtained for challenging macromolecular systems. Here we present a framework to derive the conformational sampling of isoleucine side chains from C-13 chemical shifts and demonstrate that side-chain conformations in a low-populated folding intermediate can be determined.

引用

页码：14107 / 14110

页数：4

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