Determining isoleucine side-chain rotamer-sampling in proteins from 13C chemical shift

被引：12
|
作者：
Siemons, Lucas [1 ]
Uluca-Yazgi, Boran [2 ,3 ]
Pritchard, Ruth B. [1 ]
McCarthy, Stephen [4 ]
Heise, Henrike [2 ,3 ]
Hansen, D. Flemming [1 ]
机构：
[1] UCL, Inst Struct & Mol Biol, Div Biosci, London WC1E 6BT, England
[2] Heinrich Heine Univ Dusseldorf, Inst Phys Biol, Dusseldorf, Germany
[3] Forschungszentrum Julich, Inst Complex Syst, ICS 6 Struct Biochem & JuStruct Julich Ctr Struct, Julich, Germany
[4] UCL, Dept Chem, 20 Gordon St, London WC1H 0AJ, England
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 94期
基金：
英国惠康基金; 英国医学研究理事会; 英国生物技术与生命科学研究理事会;
关键词：
DYNAMICS; COUPLINGS; BACKBONE; STATES;
D O I：
10.1039/c9cc06496f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Chemical shifts are often the only nuclear magnetic resonance parameter that can be obtained for challenging macromolecular systems. Here we present a framework to derive the conformational sampling of isoleucine side chains from C-13 chemical shifts and demonstrate that side-chain conformations in a low-populated folding intermediate can be determined.
引用
收藏
页码：14107 / 14110
页数：4
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