LYCOPENE DETERMINATION IN TOMATOES BY DIFFERENT SPECTRAL TECHNIQUES (UV-VIS, FTIR AND HPLC)

被引:0
作者
Bunghez, I. R. [1 ,2 ]
Raduly, M. [2 ]
Doncea, S. [1 ,2 ]
Aksahin, I. [3 ]
Ion, R. M. [1 ,2 ]
机构
[1] Valahia Univ, Targoviste, Romania
[2] Natl R&D Inst Chem & Petrochem, ICECHIM, Bucharest, Romania
[3] TUBITAK ATAL, Sci & Technol Res Council Turkey, Ankara Test & Anal Lab, Ankara, Turkey
关键词
Lycopene; Carotenoids; FTIR spectroscopy; INFRARED-SPECTROSCOPY;
D O I
暂无
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Lycopene is one of the most important and useful carotenoids found in nature, and a potent antioxidant that has been shown to play critical role in cancer prevention. It were studied 26 types of tomato powder samples, using different analytical methods. UV-VIS spectrophotometry showed the absorption peaks of carotenoids from these tomato powder samples in order to identify the sample with the highest lycopene concentration. FTIR spectroscopy was used to quantify lycopene content in tomatoes varieties and showed a distinct vibration band at 957 cm(-1) assigned to a trans CH deformation vibration of lycopene. The lycopene content accurately and reproducibly with a correlation coefficient of 0.9996. Results have been compared with HPLC chromatography. The outcome of the study shows that the FTIR method is a good analytical method for quantification of lycopene in tomatoes.
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页码:1349 / 1356
页数:8
相关论文
共 17 条
[1]   Prevention of oxidative stress injury to sperm [J].
Agarwal, A ;
Prabakaran, SA ;
Said, TM .
JOURNAL OF ANDROLOGY, 2005, 26 (06) :654-660
[2]   A REVIEW OF NONDESTRUCTIVE METHODS FOR QUALITY EVALUATION AND SORTING OF AGRICULTURAL PRODUCTS [J].
CHEN, P ;
SUN, Z .
JOURNAL OF AGRICULTURAL ENGINEERING RESEARCH, 1991, 49 (02) :85-98
[3]   Simultaneous quantitative determination of isolated trans fatty acids and conjugated linoleic acids in oils and fats by chernometric analysis of the infrared profiles [J].
Christy, AA ;
Egeberg, PK ;
Ostensen, ET .
VIBRATIONAL SPECTROSCOPY, 2003, 33 (1-2) :37-48
[4]   Lycopene: Chemistry, biology, and implications for human health and disease [J].
Clinton, SK .
NUTRITION REVIEWS, 1998, 56 (02) :35-51
[5]  
Giesey A.T., 1955, APPL SPECTROSC, V9, P78, DOI DOI 10.1366/000370255774634089
[6]  
Gremlich H.U., 2002, IR SPECTROSCOPY INTR
[7]  
Halim Y, 2006, J AOAC INT, V89, P1257
[8]  
Hruschka W.R., 2001, Near-Infrared Technology in the Agricultural and Food Industries, P39
[9]   Rapid determination of invert cane sugar adulteration in honey using FTIR spectroscopy and multivariate analysis [J].
Irudayaraj, J ;
Xu, F ;
Tewari, J .
JOURNAL OF FOOD SCIENCE, 2003, 68 (06) :2040-2045
[10]  
Matsuoka T., 2000, Science and Technology Research, V6, P248, DOI [DOI 10.3136/FSTR.6.248, 10.3136/fstr.6.248]