Immunonanogold-catalytic resonance scattering spectral assay of trace human chorionic gonadotrophin

被引：34

作者：

Liang, Aihui ^{[1
]}

Zou, Mingjing ^{[2
]}

Jiang, Zhiliang ^{[1
,2
]}

机构：

[1] Guilin Univ Technol, Key Lab New Proc Technol Nonferrous Metals & Mat, Minist Educ, Guilin 541004, Peoples R China

[2] Guangxi Normal Univ, Guangxi Key Lab Environm Engn Protect & Assessmen, Guilin 541004, Peoples R China

来源：

TALANTA | 2008年 / 75卷 / 05期

基金：

中国国家自然科学基金;

关键词：

human chorionic gonadotrophin; immunonanogold-catalytic silver-enhancement; silver-gold composite particles; resonance scattering spectral assay;

D O I：

10.1016/j.talanta.2008.01.015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gold nanoparticles in diameter of 10 nm were used to label rabbit anti-human chorionic gonadotrophin (RhCG) antiserum to obtain a resonance scattering spectral probe (AuRhCG) for human chorionic gonadotrophin (hCG). The immunoreaction between AuRhCG and hCG take place to form hCG-AuRhCG immunocomplex in pH 5.0 citric acid-Na2HPO4 buffer solution. The immunocomplex solutions were centrifuged to obtain the supernatant solution. The AuRhCG in the supernatant solution exhibited strong catalytic effect on the particle reaction between Ag+ and hydroquinone to produce gold-silver composite particles in pH 3.4 citric acid-trisodium citrate buffer solution. There is a stronger resonance scattering (RS) peak at 423 nm for the particles. With the addition of hCG, the AuRhCG in the supernatant solution decreased, and the RS intensity at 423 nm decreased. The decreased RS intensity Delta I-423 (nm) was proportional to the concentration of hCG in the range of 2.5-208.3 mIU/mL with a detection limit of 0.83 mIU/mL. This method has been applied to the determination of hCG in urine samples, with satisfactory results. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1214 / 1220

页数：7

共 38 条

[1] Real-time kinetic analysis of hCG-monoclonal antibody interaction using radiolabeled hCG probe: presence of two forms of Ag-mAb complex as revealed by solid phase dissociation studies [J].