A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms

被引：43
|
作者：
Li, Bin [1 ,2 ]
Comi, Troy J. [1 ,2 ]
Si, Tong [1 ,2 ,3 ,4 ]
Dunham, Sage J. B. [1 ,2 ]
Sweedler, Jonathan V. [1 ,2 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
[2] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA
[3] Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
[4] Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA
来源：
JOURNAL OF MASS SPECTROMETRY | 2016年 / 51卷 / 11期
基金：
美国国家科学基金会;
关键词：
airbrush; automatic sprayer; biofilms; MALDI MSI; matrix application; DESORPTION ELECTROSPRAY-IONIZATION; BACILLUS-SUBTILIS; MICROBIAL CULTURES; AGAR; METABOLITES; MICROSCOPY; DISCOVERY;
D O I：
10.1002/jms.3827
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Matrix-assisted laser desorption/ionization imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one-step, spray-based application of a 2,5-dihydroxybenzoic acid solution for direct matrix-assisted laser desorption/ionization imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home-built automatic sprayer, region-specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm-promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared with sieved matrix application, and is easily adapted for imaging a range of agar-based biofilms. Copyright (C) 2016 John Wiley & Sons, Ltd.
引用
收藏
页码：1030 / 1035
页数：6
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