Generic high-capacity protein capture and release by pH control

被引：19

作者：

Del Castillo, G. Ferrand-Drake ^{[1
]}

Hailes, R. L. N. ^{[1
]}

Adali-Kaya, Z. ^{[1
]}

Robson, T. ^{[1
]}

Dahlin, Andreas ^{[1
]}

机构：

[1] Chalmers Univ Technol, Dept Chem & Chem Engn, Gothenburg, Sweden

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 44期

关键词：

POLY(ACRYLIC ACID) BRUSHES; BINDING; BEHAVIOR; PAA;

D O I：

10.1039/d0cc01250e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Techniques for immobilization and release of proteins are of general interest but challenging to develop. Here we show a new method for high-capacity (several mu g cm(-2)) immobilization of proteins in polyelectrolyte brushes by multivalent hydrogen bonds. Upon increasing pH, the proteins are fully released with preserved structure and activity.

引用

页码：5889 / 5892

页数：4

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