Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation

被引:0
作者
Kim, Seul Ki [1 ,2 ]
Paik, Haerin [1 ]
Lee, Jung Ryeol [1 ,2 ]
Jee, Byung Chul [1 ,2 ,3 ]
机构
[1] Seoul Natl Univ, Bundang Hosp, Dept Obstet & Gynecol, Seongnam, South Korea
[2] Seoul Natl Univ, Coll Med, Dept Obstet & Gynecol, Seoul, South Korea
[3] Seoul Natl Univ, Bundang Hosp, Dept Obstet & Gynecol, 82 Gumi Ro 173Beon Gil, Seongnam 13620, South Korea
来源
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM | 2022年 / 49卷 / 03期
关键词
Cryopreservation; DNA fragmentation; Fertility preservation; Semen analysis; Sperm; SEMEN PARAMETERS; MEN; FERTILITY; PREGNANCY; QUALITY; IMPACT;
D O I
10.5653/cerm.2022.05323
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer.Methods: Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within-and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance.Results: The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45-0.84) and semen volume (ICC, 0.67; 95% CI, 0.45-0.84).Conclusion: Our findings showed that repeated ejaculates from patients with cancer had stable SDF levels.
引用
收藏
页码:196 / 201
页数:6
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