Development of 18 microsatellite markers for Atractylodes japonica

被引：1

作者：

Jeong, Jin-Tae ^{[1
,2
]}

Chung, Hee ^{[3
]}

Ha, Bo-Keun ^{[2
]}

Gil, Jinsu ^{[3
]}

Lee, Jeong-Hoop ^{[1
]}

Lee, Yun-Ji ^{[1
]}

Kim, Mi Ran ^{[4
]}

Oh, MyeongWon ^{[1
]}

Park, Chun Geon ^{[1
]}

Chang, Jae Ki ^{[1
]}

Hong, Chang Pyo ^{[5
]}

Park, Sin-Gi ^{[5
]}

Lee, Yi ^{[3
]}

机构：

[1] Rural Dev Adm, NIHHS, Dept Herbal Crop Res, Eumseong 27709, South Korea

[2] Chonnam Natl Univ, Div Plant Biotechnol, Gwangju 61186, South Korea

[3] Chungbuk Natl Univ, Dept Ind Plant Sci & Technol, Cheongju 28644, South Korea

[4] Fdn Agri Tech Commercializat & Transfer FACT, Dept Bioresources, Iksan 54672, South Korea

[5] Theragen Etex Bio Inst, Suwon 16229, South Korea

来源：

APPLICATIONS IN PLANT SCIENCES | 2020年 / 8卷 / 05期

关键词：

Asteraceae; Atractylodes japonica; genetic diversity; microsatellite markers; population genetics; simple sequence repeat (SSR);

D O I：

10.1002/aps3.11350

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise Atractylodes japonica (Asteraceae) is endemic to East Asia, where its rhizomes are used in traditional medicine. To investigate the genetic diversity of this species, we developed polymorphic microsatellite markers. Methods and Results We obtained a total of 175,825 simple sequence repeat (SSR) loci using the Illumina HiSeq 2500 system. Eighteen polymorphic SSR primer pairs were selected to determine heterozygosity levels and allele numbers in 80 individuals from four A. japonica populations. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.133 to 0.892, respectively. Cross-amplification in the related species A. macrocephala and A. lancea was successful in 15 and 14 of the 18 markers, respectively. Conclusions These microsatellite markers will be useful for future studies involving A. japonica population genetics and breeding.

引用

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