Promoter polymorphisms in glutathione-S-transferase genes affect transcription

被引：37

作者：

Guy, CA ^{[1
]}

Hoogendoorn, B ^{[1
]}

Smith, SK ^{[1
]}

Coleman, S ^{[1
]}

O'Donovan, MC ^{[1
]}

Buckland, PR ^{[1
]}

机构：

[1] Cardiff Univ, Coll Med, Dept Psychol Med, Cardiff CF14 4XN, S Glam, Wales

来源：

PHARMACOGENETICS | 2004年 / 14卷 / 01期

关键词：

glutathione-S-transferase; polymorphism; reporter gene assay; promoter region;

D O I：

10.1097/00008571-200401000-00005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The glutathione-S-transferases are a group of enzymes that play a major role in detoxification and defence against toxic, carcinogenic and other compounds. We analysed the proximal promoters of 14 genes encoding glutathione-S-transferase for polymorphism. Ten of the promoters contained sequence variants, nine of which we were able to clone into a reporter gene vector, pGL3. The relative ability of each haplotype to promote transcription of the luciferase gene was tested in each of two human cell lines (HEK293t and TE671) using a cotransfected CMV-SEAP plasmid as a control. Four genes (GSTA1, GSTA2, GSTM4 and GSTT2) showed activity differences greater than 1.5-fold between haplotypes, and a fifth gene (MGST1) showed a 1.4-fold difference. The promoter sequence variants in these genes may therefore play a role in human variation, susceptibility to diseases and the effects of drugs. Pharmacogenetics 14:45-51 (C) 2004 Lippincott Williams Wilkins.

引用

页码：45 / 51

页数：7

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